! NEW RESEARCH WORK !
A recent publication
by Poras H, Bonnard E, Fournié-Zaluski MC, Roques BP in "Eur J Med Chem." highlights the merits of using Bioseb's Cold Hot Plate Test
: Modulation of Disulfide Dual ENKephalinase Inhibitors (DENKIs) Activity by a Transient N-protection for Pain Alleviation by Oral Route
Bioseb’s Cold and Hot Plate Test is an innovative instrument opening new investigation fields for your analgesia research by allowing you to test animal's sensitivity to pain resulting from exposure to heat or cold
This innovative Analgesia Meter
is based on a metal plate which can be heated to 55°C and cooled to -2°C (with an ambient temperature between 20°C and 25°C). An electronic thermostat maintains the plate's temperature and a front panel digital thermometer displays the current plate temperature.
The Bioseb Cold and Hot Plate is designed to be very simple to use
and very fast to reach the set temperature (as example From ambient to 4°C, the most used threshold value, it takes less than 10 minutes, and from 4°C to 55°C it takes only 5 minutes). Metrology wise, is accurate
to less than 0,5°C (EEC metrology standard) and perfectly constant in the animal holder system. The preset temperature will not change for more than 0,1°C when a 400g rat is placed on the plate, and return to the set temperature is almost immediate.
Optional software - Screenshot
The animal's pain sensitivity resulting from exposure to heat or cold is tested by placing the animal (mouse or rat) on the surface of the plate and starting a built-in timer. The operator stops the timer at the instant the animal lifts its paw from the plate, reacting to the discomfort. The front panel timer then displays the number of seconds it took the animal to react. Animal reaction time is a measurement of animal resistance to pain and is used to measure efficacy of analgesics.
The operator can start and stop the timer with the front panel start/stop switch or with the included footswitch, which allows "hands-free" operation.
Though it is functional as a stand-alone instrument, the Cold Hot Plate Test Analgesia Meter can also be used with our dedicated optional software, allowing the user to define temperature "ramps" and “loops”. This feature is mainly used for studies with telemetry implants. In addition to displaying the reaction time, the Cold/Hot Plate Analgesia Meter is able to send the same information via USB interface to a computer.
! METHOD VALIDATION ! Publication
• Simple to use, fast and accurate
• Can be used as a stand-alone instrument
• Allows testing of sensitivity to both hot and cold stimulus
• Unmatched temperature stability and control for both heat and cold
• Fast reach to set temperatures
• Homogeneous temperature surface
• Practical foot switch timing operation
• Optional Ramp software will allow the user to define temperature ramps and loops (slope in °C/min, start and end points) and store results
Domains of application
• Analgesic drug screening
• Basal pain sensitivity phenotyping
• Integrated supraspinal responses
• Pain sensitivity alterations induced by a specific experimental context change
• Pain sensitivity alterations induced by genetic manipulations
• Animal Models of Nociception (rats and mice)
Bioseb team presents its respectful thanks to research team of Prof. Poisbeau (Institut des Neurosciences Cellulaires et Intégratives de Strasbourg), who validated the temperature ramps method used by the Cold and Hot Plate Test during in following study:
Differentiating Thermal Allodynia and Hyperalgesia Using Dynamic Hot and Cold Plate in Rodents
, by I. Yalcin, A. Charlet, MJ. Freund-Mercier, M. Barrot and P. Poisbeau, in The Journal of Pain, 2009
(Click here to download this article as a PDF file)