! NEW RESEARCH WORK !
A recent publication
by I.Mendez-David, JP. Guilloux, M. Papp, L. Tritschler, E. Mocaer, A. Gardier, S. Bretin, D. David in "Frontiers in Pharmacology" highlights the merits of using Bioseb's Elevated Plus Maze : EPM3C - 3 clicks only !
: S 47445 Produces Antidepressant- and Anxiolytic Like Effects through Neurogenesis Dependent and Independent Mechanisms
EPM3C - System overview
The Elevated Plus-Maze is a commonly used test to assess anxiety-like behavior in laboratory animals (rats/mice). Based on the natural fear that rodents have for height and open spaces, the Elevated Plus Maze is a general research tool in neurobiological anxiety field and usually uses to screen the efficacy of drugs.
The Elevated-Plus-Maze (EPM) is one of the very first tests used by neuroscientists to evaluate anxiety-like behavior in rodents and the most widely used test after the Open Field to evaluate anxiolitic or anxiogenic effects. The EPM (or its variant the Elevated-Zero-Maze) could also be considered among other various other tests for the screening of mice strains. The EPM can also be used to assess side effects of psychiatric disorders treatments.
Bioseb proposes an all-inclusive solution for the Elevated-Plus-Maze (EPM) test with hardware and software components.
Video tracking is a very good way to automate the Elevated-Plus-Maze (EPM) test for rats and mice, which is the the reason why we have designed a dedicated software application, the EPM-3C, to run any EPM/ZERO-M protocols (compatible with hardware from Bioseb or other brands), based on a powerful video tracking system. The zone transition is matched perfectly with the manual scoring by using specific morphologic detection. The tracking zones (open/closed arms, center) are really easy to define and once they are, the software is optimized to be setup easily and starts with just three clicks, in order to assist, speed up and simplify your experiments.
The Bioseb EPM Software gives all the information you need from your EPM experiment and most of the parameters are computed in real-time.
Regarding the hardware, Bioseb has designed its EPM test for mice and rats according to the standard dimensions widely used in the field. The corridors of the Bioseb Elevated-Plus-Maze are made in high quality perpex, so that the material will not retain odors. Their light grey, non-reflective color makes them ideal to combine with video-tracking. Finally, the assembly of the equipment has been made really easy.
Tripod and industrial quality camera are also provided so you can start your experiments rapidly.
EPM3C - Rat exploring - Close-up
The new EPM-3C solution from BIOSEB features unprecedented simplicity to run ELEVATED + MAZE or ZERO MAZE protocols.
The test setting consists of a plus-shaped apparatus with two open and two enclosed arms, each with an open roof and elevated from the floor. The aversion of the rodents to open spaces leads into a restriction of movement to the enclosed arms.
Anxiety of rodents is directly linked to the proportion of time spent in closed arms versus the time spent in open arms. The more anxious the animals, the more time they spend in the closed arms.
To start the test, the animal is usually placed in the center zone of the maze and the duration of the experiment is generally about 5 minutes. The time spent in the various arms is the main data recorded during this test. The Bioseb software also gives the total number of entries in the various arms (open, closed and center), the global activity of the animal (calculated with the total distance of the animal during the test) and the latency of the first entrance to the open arm.
Your results can then be exported into Excel tables for further analysis.
Supplied with :
The instrument comes with a maze, a camera, a USB cable, a boom stand and a software license.
Domains of application
• Prescreening of newly developed pharmacological agents for treatment of anxiety-related disorders
• Anxiolytic and anxiogenic effects of pharmacological agents, drugs of abuse and hormones
• Study of the effects of reproductive senescence/aging and/or pre-, peri- or postnatal exposure to various stressors
• Model to detect anxiolytic effects of benzodiazepine-related compounds
• Behavioral assay to study the brain sites (e.g., limbic regions, hippocampus, amygdala, dorsal raphe nucleus, etc.) and mechanisms (e.g., GABA, glutamate, serotonin, hypothalamic–pituitary–adrenal axis neuromodulators, etc.) underlying anxiety behavior
• Easy to set up
• Designed for Rats or Mice
• Hundreds of publication and validation for the EPM test
• The first choice for anxiety
• Modular structure which allows storage in minimum space
• Short-lasting experiment (no more than 5-10 min)
• Exploration-based conflict task
• Based on innate behavioral tendencies (ethological test)
Here is a table introducing the results available with the Bioseb software and the scientific meaning of each data :
Absolute time and experiment duration
Total time per zones (s and %)
Total time spent by the animal in open arms is the main indicator to assess anxiety
Latency to first entry per zone
Latency to first entry in open arms is another indicator to assess the anxiety of the animal
Visit (and % per zones)
Number of visits (or entries) in open arms is another indicator to assess the anxiety of the animal
Distance (cm and % per zones)
Distance covered by the animal is a good indicator of the animal activity during the test
Mean time per visits (s)
Mean time per visits in open arms can also be used as an indicator for anxiety
Mean speed (cm/s)
Speed of the animal is another indicator to assess the locomotor activity of the animal
The data is available for each arm individually, but also for grouped arms. The results can be exported on windows Excel - the software runs under Windows Seven and Eight.