! NEW RESEARCH WORK !
A recent publication
by R. Sarabia-Estrada, A. Ruiz-Valls, S. Shah, A. Ahmed, A. Ordonez, F. Rodriguez, H. Guerrero-Cazares, I. Jimenez-Estrada, E. Velarde, B. Tyler, Y. Li, N. Phillips, C. Goodwin, R. Petteys, S. Jain, G. Gallia, Z. Gokaslan, A. Quinones-Hinojosa, D. Sciubba in "Journal of Neurosurgery" highlights the merits of using Bioseb's OF3C - Automated 3D Open Field System
: Effects of primary and recurrent sacral chordoma on the motor and nociceptive function of hindlimbs in rats: an orthotopic spine model
BIOSEB designed a very innovative setup for the automation of the OPEN-FIELD TEST. While the Open field test is the most widely used test to evaluate anxiolitic or anxiogenic effects, none of the currently available systems can record horizontal and vertical activity without adding a third component such as beam brake frames. BIOSEB innovates and now offers a 3D sensor based technology accurately capable of 100% rearing detection
by direct height measurements.
The OF-3C is a dedicated software solution including the required hardware to quickly analyze spontaneous locomotion of a rodent placed in a novel environment.
BIOSEB R&D TEAM focused their efforts on designing a camera offering true 3-dimensional detection, allowing the instrument to measure the height of the highest part of the animal exploring the Open field cage.
The OF- 3C tracks the animal (rat or mouse) within the entire arena and records each REARING based on the predefined height with an accuracy of 1 mm over 1 mm2
! Our rearing detection algorithm perfectly matches the manual scoring. Zone transitions remain realistic thanks to surface thresholds and specific morphologic detection. Drawing a zone is extremely easy using our predefined zone tools : name, color, form, layer priority for complex zone shapes, etc.
The « 2 windows » software, which runs under Windows 7 and 8, computes and stores data in real time. While it can be used with any type of arena available on the market for 2D detection, all components needed for 3D detection are provided in the box.
Dedicated software and measured parameters
All parameters relevant to the Open Field test are computed by the OF-3C software in real time and can be replayed with a modified time base. The very user-friendly software allows to set up the whole test with 3 clicks in 2 windows using!
Three types of reports are generated:
: 30 parameters including distance, speed, activity duration time in zone first cross, latency first entry….
: all rearing parameters including absolute time duration, current zone, counts, etc.
: list of all transitions including the previous zone, the target zone, the transition time, the number of crossing and entries, etc.
• 3D or 2D detection
• 1 mm accuracy in height
• Minimum surface detected 1 mm2
• Eligible to sniffing detection
• Designed for Rats or Mice.
• Easy setup with just THREE CLICKS.
• Parameters are computed in real time.
Domains of application|
• Anxiolitic or anxiogenic drug screening
• Anxiety studies
• Parkinson disease
• Huntington disease
• Attention-Deficit Hyperactivity Disorder (ADHD)