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OF3C - AUTOMATED 3D OPEN FIELD SYSTEM
(Model: OF-3CM - For mice)
A brand new innovative setup for the automation of the Open Field test for rats and mice : 3D-camera based technology is now capable of direct height measurement allowing detection of 100% rearing events ! Dedicated software can bet set up in only 3 clicks to automatically assess and report global parameters, zone transitions and rearings.

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! NEW RESEARCH WORK ! A recent publication by R. Sarabia-Estrada, A. Ruiz-Valls, S. Shah, A. Ahmed, A. Ordonez, F. Rodriguez, H. Guerrero-Cazares, I. Jimenez-Estrada, E. Velarde, B. Tyler, Y. Li, N. Phillips, C. Goodwin, R. Petteys, S. Jain, G. Gallia, Z. Gokaslan, A. Quinones-Hinojosa, D. Sciubba in "Journal of Neurosurgery" highlights the merits of using Bioseb's OF3C - Automated 3D Open Field System: Effects of primary and recurrent sacral chordoma on the motor and nociceptive function of hindlimbs in rats: an orthotopic spine model

Effects of primary and recurrent sacral chordoma on the motor and nociceptive function of hindlimbs in rats: an orthotopic spine model
R. Sarabia-Estrada, A. Ruiz-Valls, S. Shah, A. Ahmed, A. Ordonez, F. Rodriguez, H. Guerrero-Cazares, I. Jimenez-Estrada, E. Velarde, B. Tyler, Y. Li, N. Phillips, C. Goodwin, R. Petteys, S. Jain, G. Gallia, Z. Gokaslan, A. Quinones-Hinojosa, D. Sciubba
Mayo Clinic Jacksonville, Florida
Published in "Journal of Neurosurgery" (2017-06-09)


OBJECTIVE

Chordoma is a slow-growing, locally aggressive cancer that is minimally responsive to conventional chemotherapy and radiotherapy and has high local recurrence rates after resection. Currently, there are no rodent models of spinal chordoma. In the present study, the authors sought to develop and characterize an orthotopic model of human chordoma in an immunocompromised rat.

METHODS

Thirty-four immunocompromised rats were randomly allocated to 4 study groups; 22 of the 34 rats were engrafted in the lumbar spine with human chordoma. The groups were as follows: UCH1 tumor-engrafted (n = 11), JHC7 tumor-engrafted (n = 11), sham surgery (n = 6), and intact control (n = 6) rats. Neurological impairment of rats due to tumor growth was evaluated using open field and locomotion gait analysis; pain response was evaluated using mechanical or thermal paw stimulation. Cone beam CT (CBCT), MRI, and nanoScan PET/CT were performed to evaluate bony changes due to tumor growth. On Day 550, rats were killed and spines were processed for H & E-based histological examination and immunohistochemistry for brachyury, S100?, and cytokeratin.

RESULTS

The spine tumors displayed typical chordoma morphology, that is, physaliferous cells filled with vacuolated cytoplasm of mucoid matrix. Brachyury immunoreactivity was confirmed by immunostaining, in which samples from tumor-engrafted rats showed a strong nuclear signal. Sclerotic lesions in the vertebral body of rats in the UCH1 and JHC7 groups were observed on CBCT. Tumor growth was confirmed using contrast-enhanced MRI. In UCH1 rats, large tumors were observed growing from the vertebral body. JHC7 chordoma-engrafted rats showed smaller tumors confined to the bone periphery compared with UCH1 chordoma-engrafted rats. Locomotion analysis showed a disruption in the normal gait pattern, with an increase in the step length and duration of the gait in tumor-engrafted rats. The distance traveled and the speed of rats in the open field test was significantly reduced in the UCH1 and JHC7 tumor-engrafted rats compared with controls. Nociceptive response to a mechanical stimulus showed a significant (p < 0.001) increase in the paw withdrawal threshold (mechanical hypalgesia). In contrast, the paw withdrawal response to a thermal stimulus decreased significantly (p < 0.05) in tumor-engrafted rats.

CONCLUSIONS

The authors developed an orthotopic human chordoma model in rats. Rats were followed for 550 days using imaging techniques, including MRI, CBCT, and nanoScan PET/CT, to evaluate lesion progression and bony integrity. Nociceptive evaluations and locomotion analysis were performed during follow-up. This model reproduces cardinal signs, such as locomotor and sensory deficits, similar to those observed clinically in human patients. To the authors' knowledge, this is the first spine rodent model of human chordoma. Its use and further study will be essential for pathophysiology research and the development of new therapeutic strategies.
Presentation

Bioseb OF3C - Open field sniffing detection
Sniffing detection
BIOSEB designed a very innovative setup for the automation of the OPEN-FIELD TEST. While the Open field test is the most widely used test to evaluate anxiolitic or anxiogenic effects, none of the currently available systems can record horizontal and vertical activity without adding a third component such as beam brake frames. BIOSEB innovates and now offers a 3D sensor based technology accurately capable of 100% rearing detection by direct height measurements.

The OF-3C is a dedicated software solution including the required hardware to quickly analyze spontaneous locomotion of a rodent placed in a novel environment.

Operating principle

BIOSEB R&D TEAM focused their efforts on designing a camera offering true 3-dimensional detection, allowing the instrument to measure the height of the highest part of the animal exploring the Open field cage.

The OF- 3C tracks the animal (rat or mouse) within the entire arena and records each REARING based on the predefined height with an accuracy of 1 mm over 1 mm2 ! Our rearing detection algorithm perfectly matches the manual scoring. Zone transitions remain realistic thanks to surface thresholds and specific morphologic detection. Drawing a zone is extremely easy using our predefined zone tools : name, color, form, layer priority for complex zone shapes, etc.

Bioseb OF3C - Open field screenshot
OF3C Screenshot
The « 2 windows » software, which runs under Windows 7 and 8, computes and stores data in real time. While it can be used with any type of arena available on the market for 2D detection, all components needed for 3D detection are provided in the box.

Dedicated software and measured parameters

All parameters relevant to the Open Field test are computed by the OF-3C software in real time and can be replayed with a modified time base. The very user-friendly software allows to set up the whole test with 3 clicks in 2 windows using!

Three types of reports are generated:

Globals: 30 parameters including distance, speed, activity duration time in zone first cross, latency first entry….
Rearings: all rearing parameters including absolute time duration, current zone, counts, etc.
Transitions: list of all transitions including the previous zone, the target zone, the transition time, the number of crossing and entries, etc.

Key features

• 3D or 2D detection
• 1 mm accuracy in height
• Minimum surface detected 1 mm2
• Eligible to sniffing detection
• Designed for Rats or Mice.
• Easy setup with just THREE CLICKS.
• Parameters are computed in real time.
Domains of application

• Anxiolitic or anxiogenic drug screening
• Anxiety studies
• Phenotyping
• Parkinson disease
• Huntington disease
• Attention-Deficit Hyperactivity Disorder (ADHD)


Publications (Click on an article to show details and read the abstract)

PAIN
- General pain -
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain. (2017)
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain.
Urien L, Gaillard S, Lo Re L, Malapert P, Bohic M, Reynders A, Moqrich A
"Aix-Marseille-Université, Institut de Biologie du Développement de Marseille, Marseille, France "
Published in "Scientific Reports" (2017-02-17)

Primary sensory neurons are heterogeneous by myriad of molecular criteria. However, the functional significance of this remarkable heterogeneity is just emerging. We precedently described the GINIP(+) neurons as a new subpopulation of non peptidergic C-fibers encompassing the free nerve ending cutaneous MRGPRD(+) neurons and C-LTMRs. Using our recently generated ginip mouse model, we have been able to selectively ablate the GINIP(+) neurons and assess their functional role in the somatosensation. We found that ablation of GINIP(+) neurons affected neither the molecular contents nor the central projections of the spared neurons. GINIP-DTR mice exhibited impaired sensation to gentle mechanical stimuli applied to their hairy skin and had normal responses to noxious mechanical stimuli applied to their glabrous skin, under acute and injury-induced conditions. Importantly, loss of GINIP(+) neurons significantly altered formalin-evoked first pain and drastically suppressed the second pain response. Given that MRGPRD(+) neurons have been shown to be dispensable for formalin-evoked pain, our study suggest that C-LTMRs play a critical role in the modulation of formalin-evoked pain.

- Mechanical allodynia & hyperlagesia -
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain. (2017)
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain.
Urien L, Gaillard S, Lo Re L, Malapert P, Bohic M, Reynders A, Moqrich A
"Aix-Marseille-Université, Institut de Biologie du Développement de Marseille, Marseille, France "
Published in "Scientific Reports" (2017-02-17)

Primary sensory neurons are heterogeneous by myriad of molecular criteria. However, the functional significance of this remarkable heterogeneity is just emerging. We precedently described the GINIP(+) neurons as a new subpopulation of non peptidergic C-fibers encompassing the free nerve ending cutaneous MRGPRD(+) neurons and C-LTMRs. Using our recently generated ginip mouse model, we have been able to selectively ablate the GINIP(+) neurons and assess their functional role in the somatosensation. We found that ablation of GINIP(+) neurons affected neither the molecular contents nor the central projections of the spared neurons. GINIP-DTR mice exhibited impaired sensation to gentle mechanical stimuli applied to their hairy skin and had normal responses to noxious mechanical stimuli applied to their glabrous skin, under acute and injury-induced conditions. Importantly, loss of GINIP(+) neurons significantly altered formalin-evoked first pain and drastically suppressed the second pain response. Given that MRGPRD(+) neurons have been shown to be dispensable for formalin-evoked pain, our study suggest that C-LTMRs play a critical role in the modulation of formalin-evoked pain.

SENSORY SYSTEM
- Somatosensory system -
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain. (2017)
Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain.
Urien L, Gaillard S, Lo Re L, Malapert P, Bohic M, Reynders A, Moqrich A
"Aix-Marseille-Université, Institut de Biologie du Développement de Marseille, Marseille, France "
Published in "Scientific Reports" (2017-02-17)

Primary sensory neurons are heterogeneous by myriad of molecular criteria. However, the functional significance of this remarkable heterogeneity is just emerging. We precedently described the GINIP(+) neurons as a new subpopulation of non peptidergic C-fibers encompassing the free nerve ending cutaneous MRGPRD(+) neurons and C-LTMRs. Using our recently generated ginip mouse model, we have been able to selectively ablate the GINIP(+) neurons and assess their functional role in the somatosensation. We found that ablation of GINIP(+) neurons affected neither the molecular contents nor the central projections of the spared neurons. GINIP-DTR mice exhibited impaired sensation to gentle mechanical stimuli applied to their hairy skin and had normal responses to noxious mechanical stimuli applied to their glabrous skin, under acute and injury-induced conditions. Importantly, loss of GINIP(+) neurons significantly altered formalin-evoked first pain and drastically suppressed the second pain response. Given that MRGPRD(+) neurons have been shown to be dispensable for formalin-evoked pain, our study suggest that C-LTMRs play a critical role in the modulation of formalin-evoked pain.

CROSS-DISCIPLINARY SUBJECTS
- Cancer -
Effects of primary and recurrent sacral chordoma on the motor and nociceptive function of hindlimbs in rats: an orthotopic spine model (2017)
Effects of primary and recurrent sacral chordoma on the motor and nociceptive function of hindlimbs in rats: an orthotopic spine model
R. Sarabia-Estrada, A. Ruiz-Valls, S. Shah, A. Ahmed, A. Ordonez, F. Rodriguez, H. Guerrero-Cazares, I. Jimenez-Estrada, E. Velarde, B. Tyler, Y. Li, N. Phillips, C. Goodwin, R. Petteys, S. Jain, G. Gallia, Z. Gokaslan, A. Quinones-Hinojosa, D. Sciubba
Mayo Clinic Jacksonville, Florida
Published in "Journal of Neurosurgery" (2017-06-09)

OBJECTIVE

Chordoma is a slow-growing, locally aggressive cancer that is minimally responsive to conventional chemotherapy and radiotherapy and has high local recurrence rates after resection. Currently, there are no rodent models of spinal chordoma. In the present study, the authors sought to develop and characterize an orthotopic model of human chordoma in an immunocompromised rat.

METHODS

Thirty-four immunocompromised rats were randomly allocated to 4 study groups; 22 of the 34 rats were engrafted in the lumbar spine with human chordoma. The groups were as follows: UCH1 tumor-engrafted (n = 11), JHC7 tumor-engrafted (n = 11), sham surgery (n = 6), and intact control (n = 6) rats. Neurological impairment of rats due to tumor growth was evaluated using open field and locomotion gait analysis; pain response was evaluated using mechanical or thermal paw stimulation. Cone beam CT (CBCT), MRI, and nanoScan PET/CT were performed to evaluate bony changes due to tumor growth. On Day 550, rats were killed and spines were processed for H & E-based histological examination and immunohistochemistry for brachyury, S100?, and cytokeratin.

RESULTS

The spine tumors displayed typical chordoma morphology, that is, physaliferous cells filled with vacuolated cytoplasm of mucoid matrix. Brachyury immunoreactivity was confirmed by immunostaining, in which samples from tumor-engrafted rats showed a strong nuclear signal. Sclerotic lesions in the vertebral body of rats in the UCH1 and JHC7 groups were observed on CBCT. Tumor growth was confirmed using contrast-enhanced MRI. In UCH1 rats, large tumors were observed growing from the vertebral body. JHC7 chordoma-engrafted rats showed smaller tumors confined to the bone periphery compared with UCH1 chordoma-engrafted rats. Locomotion analysis showed a disruption in the normal gait pattern, with an increase in the step length and duration of the gait in tumor-engrafted rats. The distance traveled and the speed of rats in the open field test was significantly reduced in the UCH1 and JHC7 tumor-engrafted rats compared with controls. Nociceptive response to a mechanical stimulus showed a significant (p < 0.001) increase in the paw withdrawal threshold (mechanical hypalgesia). In contrast, the paw withdrawal response to a thermal stimulus decreased significantly (p < 0.05) in tumor-engrafted rats.

CONCLUSIONS

The authors developed an orthotopic human chordoma model in rats. Rats were followed for 550 days using imaging techniques, including MRI, CBCT, and nanoScan PET/CT, to evaluate lesion progression and bony integrity. Nociceptive evaluations and locomotion analysis were performed during follow-up. This model reproduces cardinal signs, such as locomotor and sensory deficits, similar to those observed clinically in human patients. To the authors' knowledge, this is the first spine rodent model of human chordoma. Its use and further study will be essential for pathophysiology research and the development of new therapeutic strategies.


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Technology Color tracking+ 3D technology
Supported OS see our software page for details
Analysis Real-time from tracking and 3 D camera
Camera Include - HD USB based
Maze Included - Grey PVC, to be chosen between 2 versions: rats or mice
Stand Included
Dimensions - For rats Inner dimensions: L 1000 mm / W 1000 mm / H 400 mm
Total dimensions : L 1082 mm / W 1050 mm / H 1770 mm
Weight: 32 kg
Dimensions - For mice Inner dimensions:L 400 mm / W 400 mm / H 400 mm
Total dimensions: L 472 mm / W 440 mm / H 930 mm
Weight: 17 kg
Colors Arena RAL 7032 / Camera RAL 9005

Model:
OF-3CM
OF3C - Automated 3D Open Field System (Modif.)
For mice Contact us

Related products:
OF-3CR
For rats Contact us
OF-3CB
Software license only Contact us
Print version

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