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ACTIVMETER
(Model: BIO-ACTIV-M - For Mice)
A new generation of activity meter: based on vibrations analysis ONLY, Bioseb's ACTIVMETER measures Activity and Locomotion of rodents (mouse and rat) in their home cage. An accurate and economical instrument

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  • UNIVERSITY OF PITTSBURG Pittsburg, USA
  • INSTITUT DE BIOLOGIE DU DEVELOPEMENT DE MARSEILLE LUMINY Marseille,France
  • FACULTÉ DE MÉDECINE Clermont-Ferrand ,France
  • INSTITUT DE MYOLOGIE, HOPITAL PITIE SALPETRIERE Paris,France
  • INSERM INSTITUT COCHIN Paris,France
  • FACULTE DE MÉDECINE St Etienne,France
  • INSERM, UNIVERSITE DE MONTPELLIER Montpellier,France
  • UNIVERSTITE DE FRANCHE COMPTE Besancon,France
  • GENETHON Evry,France
  • INSERM,HOPITAL ROBERT DEBRE Paris, France
  • CNRS UNIVERSITE CLAUDE BERNARD Villeurbanne, France
  • EA 3544, UNIVERSITE PARIS SUD Châtenay-Malabry, France
  • CNRS UMR 7102, UPMC Paris, France
  • INSTITUT DE RECHERCHE PIERRE FABRE Castres,France
  • UNIVERSITE DE LAUSANNE, DPT ANESTHESIOLOGIE Lausanne, Suisse
  • GENETHON Evry,France
  • ECOLE VETERINAIRE, PHARMACOLOGIE Nantes,France
  • PLATE-FORME ANIPHY, FACULTE ROCKEFELLER Lyon, France
  • UNIVERSITE DE METZ Metz, France
  • UNIVERSITY OF IOWA USA
  • VIRGINIA TECH Roanoke, USA
  • INSERM GH PITIE SALPETRIERE Paris, France
  • UNIVERSITY MCGILL Montreal, Canada
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! NEW RESEARCH WORK ! A recent publication by A. Charlet, J.-L. Rodeau, P. Poisbeau. in "The Journal of Pain" highlights the merits of using Bioseb's Activmeter: Radiotelemetric and Symptomatic Evaluation of Pain in the Rat After Laparotomy: Long-Term Benefits of Perioperative Ropivacaine Care.

Radiotelemetric and Symptomatic Evaluation of Pain in the Rat After Laparotomy: Long-Term Benefits of Perioperative Ropivacaine Care.
A. Charlet, J.-L. Rodeau, P. Poisbeau.
Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives,Nociception and Pain Department, Strasbourg, France.
Published in "The Journal of Pain" (2011-02-28)


Effective relief of acute and long-term postoperative pain is of utmost importance to patients undergoing surgery. Here, we worked on a controlled procedure of abdominal surgery in the rat inducing persistent postoperative pain symptoms for up to 10 days and tested the efficacy of perioperative care with the local anesthetic ropivacaine. Laparotomy was likewise used to implant radiotelemetric probes by which electrocardiogram, body temperature, and locomotor activity were recorded in freely moving animals. We showed that postoperative pain symptoms (mechanical allodynia) measured in periphery of the scar were associated over time with persistent tachycardia, elevated heart rate variability, and loss of mobility. Furthermore, a single subcutaneous infiltration of the local anesthetic ropivacaine in the periphery of the abdominal incision was sufficient to prevent the appearance of allodynia and the associated cardiac and motor signs of pain, monitored by radiotelemetry. These beneficial effects were observed when the infiltration was performed in the perioperative period, but not later. This study on freely moving animals exhibiting long-lasting postoperative pain symptoms and altered autonomic/motor function illustrates well the importance of the timing of preemptive analgesia care with long-acting local anesthetics. Moreover, it emphasizes the utility of monitoring heart rate variability to quantify spontaneous expression of long-lasting postoperative pain. PERSPECTIVE: Speeding the recovery time after surgery using perioperative ropivacaine care is of significant clinical relevance because it might limit the risk of chronic pain and postoperative complications. In humans, chronobiological analysis of heart rate variability could also help quantify spontaneous pain expression with minimal emotional bias.

Presentation

Activ-Meter by Bioseb: a new way of assessing rodent's activity in their home cage, for mice and rats
Activmeter with extra IR-sensors
Bioseb has developed a really simple-to-use and miniature actimetry system. The instrument uses vibrations from within the cage to measure the locomotion and includes exhaustive functions to quantify rodent activity over short or long periods of time. It is usually set over 24 hours to quantify the activity over a single circadian cycle.

With a very competitive pricing for 1 to 8 cages, it is a "must" when you need to simply quantify the locomotor phenotype of your mice and rats. The ACTIVMETER allows the accurate measurement of the rodent's activity in its familiar environment using the animal's ‘Home Cage' (including grid, pellets and bottle), and it can also be used in any other kind of environment (open field, etc.).

Operating principle

Very different from other techniques, our new actimeter "ActiV-Meter" does not require any kind of surgical implantation on the animal (mouse or rat), does not involve any kind of modification to the home cage, and is not disturbed by any change (like litter movements) in the cage environment. Other actimeter systems, like the Infra-red beams and the video image analysis monitoring, are really not usable with litter, not very accurate in spatial definition and cannot detect small movements and subtle behaviors, e.g. the “tremor”. Unlike them, the ActiV-Meter is using an actimetry technique similar to the force based actometers, but does not require the use of specific cages and statistical algorithms which make other activity measurement systems very expensive to set up and use in daily practice.

Key features

Activ-Meter by Bioseb: measuring 'Rearing' activity of a mouse in the home cage The originality of the ACTIVMETER from Bioseb lies in its ability to quantify 3 parameters of animal behaviour:
• Movement
• Motionless Activity (including tremor)
• Immobility

Among other advantages, this actimeter is characterized by its ease of use, the fact that the activity measurement can take place in the Home Cage as well as in Open Field, and that the animal, mouse or rat, is unrestrained during the experiment. Using food and a bottle, activity measurement experiments can be conducted over a very long period of time.

In addition to these features, Bioseb's ACTIVMETER incorporates a very unique benefit by also measuring the activity when the animal walks or hangs under the wire mesh cover grid (especially useful for mice), giving a true idea of the aptitude and ability of the mice to navigate under a wire mesh grid. The assessment of this aptitude might suggest further evaluation of sensorimotor abilities of the model, using classical tests like the rotarod, the grip test, etc.

The operator of the actometer can define his own immobility standard as well as the thresholds between slow motion and fast motion. During the actimetry experiment, the operator can visualize on the screen the real-time activity type, position and motion, as well as the parameters measured during each sampling period for each animal (mouse or rat). The operator can also mark special behaviour types using a notepad application and keyboard shortcuts (ethological keyboard). Raw acquisition data files are stored in a proprietary format to meet GLP standards, while a .txt version can be used for further activity analysis.

All of this at a fraction of the usual price of other actimeter systems and with a much easier setup.

Measured Parameters

Results can be sent directly to MS Excel and/or exported in .txt format. Measured parameters are:
• Immobility time (sleeping or freezing periods), defined with a specific threshold
• Motionless activity duration (the animal is awake but not moving in the cage, for example while eating or scratching…)
• Animal movement: distance (given in cm) and duration, possibility to split between slow motion and fast motion duration. Also available when the mice navigate under a wire mesh grid
• Average speed of the animal (mouse or rat), calculated using moving period of time
• Rearing can be detected and counted especially useful when working in an open field. The ACTIVMETER can count the number of "rearings" and measure the cumulative time
• All parameters can be changed by the operator and the experiment replayed later using different threshold values.

Domains of application

Bioseb’s new actimeter for rodents (rats and mice) is especially useful for following applications and research fields:
• General phenotyping
• Neurodegenerative diseases
• Myopathy diseases
• Global locomotor activity associated to radio telemetric measurements
• Studies on Circadian rhythms and associated troubles
• Models used to study psychiatric diseases such as STOP null mice showing disorganized activity and hypersensitivity to stressed event
• Recovery on partial SCI models
• And much more...

Bioseb's new actimetry software for the Activ-Meter
BIO-ACTIV Software for actimetry data acquisition and data replay


!New! Publication:

Bioseb team presents its respectful thanks to research teams of Prof. David (Université Paris-Sud) and Prof. Samuels (Columbia University, New York), who used the Activ_Meter (Actimeter for Rodents) during their recent studies:

Behavioral Effects of Fluoxetine in an Animal Model of Anxiety/Depression Are Mediated by Both Neurogenesis-Dependent and -Independent Mechanisms, by DJ. David, BA. Samuels, Q. Rainer, JW. Wang et al., in Neuron, 62, 2009
(Click here to download this article as a PDF file)

Publications (Click on an article to show details and read the abstract)

PAIN
- General pain -
Radiotelemetric and Symptomatic Evaluation of Pain in the Rat After Laparotomy: Long-Term Benefits of Perioperative Ropivacaine Care. (2011)
Radiotelemetric and Symptomatic Evaluation of Pain in the Rat After Laparotomy: Long-Term Benefits of Perioperative Ropivacaine Care.
A. Charlet, J.-L. Rodeau, P. Poisbeau.
Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives,Nociception and Pain Department, Strasbourg, France.
Published in "The Journal of Pain" (2011-02-28)

Effective relief of acute and long-term postoperative pain is of utmost importance to patients undergoing surgery. Here, we worked on a controlled procedure of abdominal surgery in the rat inducing persistent postoperative pain symptoms for up to 10 days and tested the efficacy of perioperative care with the local anesthetic ropivacaine. Laparotomy was likewise used to implant radiotelemetric probes by which electrocardiogram, body temperature, and locomotor activity were recorded in freely moving animals. We showed that postoperative pain symptoms (mechanical allodynia) measured in periphery of the scar were associated over time with persistent tachycardia, elevated heart rate variability, and loss of mobility. Furthermore, a single subcutaneous infiltration of the local anesthetic ropivacaine in the periphery of the abdominal incision was sufficient to prevent the appearance of allodynia and the associated cardiac and motor signs of pain, monitored by radiotelemetry. These beneficial effects were observed when the infiltration was performed in the perioperative period, but not later. This study on freely moving animals exhibiting long-lasting postoperative pain symptoms and altered autonomic/motor function illustrates well the importance of the timing of preemptive analgesia care with long-acting local anesthetics. Moreover, it emphasizes the utility of monitoring heart rate variability to quantify spontaneous expression of long-lasting postoperative pain. PERSPECTIVE: Speeding the recovery time after surgery using perioperative ropivacaine care is of significant clinical relevance because it might limit the risk of chronic pain and postoperative complications. In humans, chronobiological analysis of heart rate variability could also help quantify spontaneous pain expression with minimal emotional bias.

- Inflammatory pain -
ASIC3 deficiency increases inflammation but decreases pain behavior in arthritis (2013)
ASIC3 deficiency increases inflammation but decreases pain behavior in arthritis
KA. Sluka, LA. Rasmussen, MM. Edgar, JM. O'Donnell, RY. Walder et al
Department of Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa, Iowa City, United States of America
Published in "Arthritis & Rheumatism" (2013-01-17)

Objective.
Through its location on nociceptors, acid sensing ion channel 3 (ASIC3) is activated by decreases in pH and plays a significant role in musculoskeletal pain. We recently showed that decreases in pH activate ASIC3 located on fibroblast-like synoviocytes (FLS). Since FLS are key cells in the inflammatory process we tested if ASIC3-deficient mice with arthritis have altered inflammation and pain relative to controls.
Methods.
Arthritis was induced by injection of a cocktail of anti-type II collagen antibodies induced collagen antibodyarthritis (CAIA). Inflammation and pain parameters in ASIC3-/- and ASIC3+/+ mice were assessed. Disease severity was measured with clinical arthritis scores, joint diameters, histological analysis of joints, and qPCR for synovial gene expression. Pain behaviors were measured by examining withdrawal thresholds of the joint and paw and by measuring physical activity levels in mice. Cell death was assessed with a Live/Dead assay in FLS in response to decreases in pH.
Results.
Surprisingly, ASIC3-/- mice with CAIA demonstrated significantly increased joint inflammation, joint destruction and expression of IL-6, MMP-3 and MMP-13 in joint tissue compared to ASIC3+/+ mice. ASIC3+/+ FLS show enhanced cell death when exposed to pH 6.0 in the presence of interleukin-1β that is abolished in ASIC3-/- FLS. Despite enhanced disease severity, ASIC3-/- mice do not develop mechanical hypersensitivity of the paw and show greater levels of physical activity.
Conclusion.
These data are consistent with the hypothesis that ASIC3 plays a protective role in inflammatory arthritis conditions by limiting inflammation through enhanced synoviocyte cell death to reduce disease severity and produce pain to reduce joint use.

CENTRAL NERVOUS SYSTEM (CNS)
- Ischemic Strokes -
Intramuscular scAAV9-SMN injection mediates widespread gene delivery to the spinal cord and decreases disease severity in SMA mice (2013)
Intramuscular scAAV9-SMN injection mediates widespread gene delivery to the spinal cord and decreases disease severity in SMA mice
S. Benkhelifa-Ziyyat, A. Besse, M. Roda, S. Duque, S. Astord et al
CNRS UMR 7215, Institut de Myologie, Université Pierre et Marie Curie, Paris, France
Published in "Molecular Therapy" (2013-02-28)

We have recently demonstrated the remarkable efficiency of self-complementary (sc) AAV9 vectors for central nervous system (CNS) gene transfer following intravenous delivery in mice and larger animals. Here, we investigated whether gene delivery to motor neurons (MNs) could also be achieved via intramuscular (i.m.) scAAV9 injection and subsequent retrograde transport along the MNs axons. Unexpectedly, we found that a single injection of scAAV9 into the adult mouse gastrocnemius (GA) mediated widespread MN transduction along the whole spinal cord, without limitation to the MNs connected to the injected muscle. Spinal cord astrocytes and peripheral organs were also transduced, indicating vector spread from the injected muscle to both the CNS and the periphery through release into the blood circulation. Moreover, we showed that i.m. injection of scAAV9 vectors expressing “survival of motor neuron” (Smn) in spinal muscular atrophy (SMA) mice mediated high survival motor neuron (SMN) expression levels at both the CNS and the periphery, and increased the median lifespan from 12 days to 163 days. These findings represent to date the longest extent in survival obtained in SMA mice following i.m. viral vector gene delivery, and might generate a renewed interest in the use of i.m. adeno-associated viruses (AAV) delivery for the development of gene therapy strategies for MN diseases.

NEURODEGENERATION
- Parkinson disease -
Progressive Parkinsonism by acute dysfunction of excitatory amino acid transporters in the rat substantia nigra (2014)
Progressive Parkinsonism by acute dysfunction of excitatory amino acid transporters in the rat substantia nigra
M. Assousa, L. Had-Aissounia, P. Gubellinia, C. Melona, I. Nafia et al.
Aix-Marseille Université, Marseille, France
Published in "Neurobiology of Disease" (2014-01-27)

Parkinson's disease (PD) is characterized by the progressive degeneration of substantia nigra (SN) dopamine neurons, involving a multifactorial cascade of pathogenic events. Here we explored the hypothesis that dysfunction of excitatory amino acid transporters (EAATs) might be involved. Acutely-induced dysfunction of EAATs in the rat SN, by single unilateral injection of their substrate inhibitor l-trans-pyrrolidine-2,4-dicarboxylate (PDC), triggers a neurodegenerative process mimicking several PD features. Dopamine neurons are selectively affected, consistent with their sustained excitation by PDC measured by slice electrophysiology. The anti-oxidant N-acetylcysteine and the NMDA receptor antagonists ifenprodil and memantine provide neuroprotection. Besides oxidative stress and NMDA receptor-mediated excitotoxicity, glutathione depletion and neuroinflammation characterize the primary insult. Most interestingly, the degeneration progresses overtime with unilateral to bilateral and caudo-rostral evolution. Transient adaptive changes in dopamine function markers in SN and striatum accompany cell loss and axonal dystrophy, respectively. Motor deficits appear when neuron loss exceeds 50% in the most affected SN and striatal dopamine tone is dramatically reduced. These findings outline a functional link between EAAT dysfunction and several PD pathogenic mechanisms/pathological hallmarks, and provide a novel acutely-triggered model of progressive Parkinsonism.

MOOD DISORDERS
- Anxiety -
Beneficial behavioural and neurogenic effects of agomelatine in a model of depression/anxiety. (2011)
Beneficial behavioural and neurogenic effects of agomelatine in a model of depression/anxiety.
Q. Rainer, L. Xia, JP. Guilloux, C. Gabriel, DJ. David et al
Université Paris-Sud, Laboratoire Neuropharmacologie, Châtenay-Malabry, France.
Published in "International journal of neuropsychology" (2011-01-26)

Agomelatine (S20098) is a novel antidepressant drug with melatonergic agonist and 5-HT2C receptor antagonist properties, displaying antidepressant/anxiolytic-like properties in animal models and in humans. In a depression/anxiety-like mouse model in which the response of the HPA axis is blunted, we investigated whether agomelatine could reverse behavioural deficits related to depression/anxiety compared to the classical selective serotonin reuptake inhibitor, fluoxetine. Adult mice were treated for 8 wk with either vehicle or corticosterone (35 μg/ml.d) via drinking water. During the final 4 wk, animals were treated with vehicle, agomelatine (10 or 40 mg/kg i.p.) or fluoxetine (18 mg/kg i.p.) and tested in several behavioural paradigms and also evaluated for home-cage activity. Our results showed that the depressive/anxiety-like phenotype induced by corticosterone treatment is reversed by either chronic agomelatine or fluoxetine treatment. Moreover, agomelatine increased the dark/light ratio of home-cage activity in vehicle-treated mice and reversed the alterations in this ratio induced by chronic corticosterone, suggesting a normalization of disturbed circadian rhythms. Finally, we investigated the effects of this new antidepressant on neurogenesis. Agomelatine reversed the decreased cell proliferation in the whole hippocampus in corticosterone-treated mice and increased maturation of newborn neurons in both vehicle- and corticosterone-treated mice. Overall, the present study suggests that agomelatine, with its distinct mechanism of action based on the synergy between the melatonergic agonist and 5-HT2C antagonist properties, provides a distinct antidepressant/anxiolytic spectrum including circadian rhythm normalization.

- Depression -
Beneficial behavioural and neurogenic effects of agomelatine in a model of depression/anxiety. (2011)
Beneficial behavioural and neurogenic effects of agomelatine in a model of depression/anxiety.
Q. Rainer, L. Xia, JP. Guilloux, C. Gabriel, DJ. David et al
Université Paris-Sud, Laboratoire Neuropharmacologie, Châtenay-Malabry, France.
Published in "International journal of neuropsychology" (2011-01-26)

Agomelatine (S20098) is a novel antidepressant drug with melatonergic agonist and 5-HT2C receptor antagonist properties, displaying antidepressant/anxiolytic-like properties in animal models and in humans. In a depression/anxiety-like mouse model in which the response of the HPA axis is blunted, we investigated whether agomelatine could reverse behavioural deficits related to depression/anxiety compared to the classical selective serotonin reuptake inhibitor, fluoxetine. Adult mice were treated for 8 wk with either vehicle or corticosterone (35 μg/ml.d) via drinking water. During the final 4 wk, animals were treated with vehicle, agomelatine (10 or 40 mg/kg i.p.) or fluoxetine (18 mg/kg i.p.) and tested in several behavioural paradigms and also evaluated for home-cage activity. Our results showed that the depressive/anxiety-like phenotype induced by corticosterone treatment is reversed by either chronic agomelatine or fluoxetine treatment. Moreover, agomelatine increased the dark/light ratio of home-cage activity in vehicle-treated mice and reversed the alterations in this ratio induced by chronic corticosterone, suggesting a normalization of disturbed circadian rhythms. Finally, we investigated the effects of this new antidepressant on neurogenesis. Agomelatine reversed the decreased cell proliferation in the whole hippocampus in corticosterone-treated mice and increased maturation of newborn neurons in both vehicle- and corticosterone-treated mice. Overall, the present study suggests that agomelatine, with its distinct mechanism of action based on the synergy between the melatonergic agonist and 5-HT2C antagonist properties, provides a distinct antidepressant/anxiolytic spectrum including circadian rhythm normalization.



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Overall Dimensions (Lx W x H cm) and Weight (g) 23 x 14 x 5, 1950 g
Power supply Power adapter 110/220V
Number of Activmeters per PC Up to 8
Sensor calibration / accuracy User calibration
Sampling rate 100 Hz
Spatial accuracy : 1 cm default for mice / 2.5 cm default for rats
Activmeter for Mice Recommended Home cage : Eurocage Type II
Max. weight cages up to 2 000g for animals heavier than 17g
Activmeter for Rats Recommended Home cage : Eurocage Type III
Max. weight cages up to 4 000g for animals of less than 500g.
Delivered with Software BIO-ACTIV to monitor up to 8 different cages simultaneously / Mouse management and data management included / Raw datas with sampling rate 100Hz, encrypted (glp) averaged and recorded results directly exported in xls or txt files / Complete file describing the animal position and behavior status / User defined timing of data presentation from 1 second to days / Replay and control: at any time, remotly / All experiments can be re-analysed at user will, analysis of animal – groups , and results gathered by protocols…
Components included For mice : Eurostandard Type IIL cage from ERHET and its dedicated TRAY
For rats: generic TRAY that fits most on the cages from the market.
PC Requirement for software 2GO RAM, Fast processor, Windows 7, USB port
Option Rearing accessories and cages, Home cage, Open field, mazes, behavior experiment cages.
Contact Bioseb for more information

Model:
BIO-ACTIV-M
Activmeter (Modif.)
For Mice Contact us

Related products:
BIO-ACTIV-R
For rats Contact us
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