! NEW RESEARCH WORK !
A recent publication
by Perlikowska R, Piekielna J, Mazur M, Koralewski R, Olczak J, do Rego JC, Fichna J, Modranka J, Janecki T, Janecka A in "Bioorg Med Chem." highlights the merits of using Bioseb's Forced Swimming Test: New FST DUAL SENSOR
: Antinociceptive and antidepressant-like action of endomorphin-2 analogs with proline surrogates in position 2
The Forced Swimming Test (FST), also known as Porsolt Swimming Test
or "behavioral despair test", is a common behavioral test for the development and screening of antidepressant and anxiolytic drugs
to identify new treatments and understand the biological mechanisms of current treatments.
Bioseb's Forced Swimming Test FST DUAL SENSOR has been designed for rats and mice thanks to the expert work of Dr. Denis DAVID (described in a publication in NEURON in 2009, "Neurogenesis Dependent and Independent Effects of Fluoxetine in an Animal Model of Anxiety/Depression").
What is the TYC algorithm of Bioseb's new Forced Swimming Test ?
• A new tool that gives the researcher total control when evaluating protocols.
• The researcher is the expert!
• The TYC is taught how to detect the 3 states: Resting/Floating, Swimming, and Climbing/Struggling
The scoring can be learned and then consistently applied with no variability and drift over time! The TYC algorithm is an optimization tool: with a single run manually evaluated by the researcher, one will train the software to use scored parameters, TYC will adjust scoring algorithms to follow the researcher's evaluation rules.
The system allows easy comparison of several approaches, useful in training students, and provides consistency across multiple studies
Two types of behavior can be observed during the Porsolt Test using FST DUAL SENSOR:
1. Non-depressed rodents (rats or mice), even when unable to escape from the water-filled cylinders, will try to swim and struggle to escape the containers.
2. Depressed rodents will stop trying sooner than non-depressed rodents and start to float in the cylinders, showing behavioural despair.
The floating or immobility time during the forced swimming test is an accurate indication of the effects of anti-depressants and anxiolitics.
Bioseb offers a powerful dual approach system based on vibrations detection and video-based motor activity analysis
for automating the Porsolt Forced Swimming Test. Using the state-of-the-art video tracking algorithms combined with each beaker vibration, the software solution allows the researcher to analyse and separate different behaviours via a calibration process that learned from an expert in the lab.
Up to 4 animals (mouse or rat) can be tested simultaneously. A manual set-up would only allow the assessment of 1 animal at a time.
Using the FST DUAL SENSOR, the researcher will be able to record video of the trials, calculate the immobility time, swimming time, and struggling time for each animal tested and do so in batches of four
. Each acquisition can be replayed and re-analyzed later with different settings : thus, a calibration made by an expert from another lab could be used as well. Results can be exported to any software of choice for further analysis.
The management of the Porsolt Forced Swimming Test experiment is facilitated via a list of animals that can be entered into the software or imported from excel. Several possibilities for randomization are presented, and the randomized list can be exported, imported, and printed. Runs can be paused or started at any time.
Bioseb's Forced Swimming Test (Porsolt): New FST DUAL SENSOR - Software screen-shots
The BIOSEB Forced Swimming Test FST DUAL SENSOR solution allow automatic computing of three parameters or states for the rats and the mice:
• Non-active (immobility, passive floating)
• Dipping (rat only) which can be easily set manually when analysing the files
Results are organized by groups or individual animal, and can be displayed for every minute of the test.
Domains of application
• Review of antidepressant activity
• Primary screening test for antidepressants
• Evaluation of psycho-stimulants
• Evaluation of anxiety treatments
Examples of Agents used are Imiprimine, Fluoxetine, Desipramine, and Venlafaxine.
• BIO-FST-BKR Instrumented Spare beaker for rats (30 cm diameter x 50 cm height)
• BIO-FST-BKM Instrumented Spare beaker for mice (17 cm diameter x 20 cm height)
• A customized support frame that separates the four beakers, houses the interface and supports the HD camera
• Four graduated beakers for rats or mice, (30 cm diameter for rats, 17 cm diameter for mice; extras available upon request)
• An FST DUAL SENSOR software product with license
• User's manual