Inula Japonica Thunb- Flower Ethanol Extract Improves Obesity and Exercise Endurance in Mice Fed A High-Fat Diet

Authors
SH Park, DH Lee, MJ Kim, J Ahn, YJ Jang, TY Ha

Lab
Department of Food Biotechnology, Korea University of Science and Technology, Wanju-gun, Jeollabuk-do 55365, Korea

Journal
Nutrients

Abstract
Inula japonica Thunb. (Asteraceae) is a _owering plant that grows mainly in Korea, Japan, and China and its _ower extract has diverse biological effects such as anti-in_ammatory and antioxidative activities. However, the effects on obesity and enhancement of endurance capacity have not been explored yet. This study aims to reveal the effects of I. japonica _ower ethanol extract (IJE)onobesityandendurancecapacityinhigh-fatdiet(HFD)fedC57BL/6Jmiceandthemechanism. IJE inhibited lipid accumulation in 3T3-L1 adipocytes in vitro. Also, IJE-fed mice showed reduced body weight gain, hepatic lipid, and body fat mass, and increased muscle weight. IJE reduced lipid accumulation in the liver and adipose tissue by decreasing lipogenic and adipogenic gene expression. Additionally,consumptionoflow-doseIJEsigni_cantlyenhancedendurancecapacityvia increasing AMP-activated protein kinase activity and mRNA levels of Myh7 and Myh2. Luteolin and 1_-hydroxyalantolactone (1_-HA), compounds of IJE, are involved in anti-adipogenesis in the 3T3-L cells and only luteolin increased the protein levels of MHC during C2C12 myoblast differentiation. Collectively, our results suggest that consumption of IJE not only helps to prevent obesity but also enhances endurance capacity reduced by HFD.

BIOSEB Instruments Used:
Grip strength test (BIO-GS3)