Assessment of ICU?acquired weakness in young and old mice- an E- coli septic peritonitis model

Authors
Witteveen E, Hoogland IC, Wieske L, Weber NC, Verhamme C, Schultz MJ, van Schaik IN, Horn J

Lab
Department of Intensive Care Medicine, Academic Medical Center, Amsterdam, the Netherlands.

Journal
Muscle Nerve.

Abstract
Introduction There are few reports of in vivo muscle strength measurements in animal models of ICU-acquired weakness (ICU-AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU-AW model using in vivo strength measurements and myosin-actin assays, and whether development of ICU-AW is age-dependent in this model. Methods Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and myosin-actin ratio in muscle was determined. Results E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin-actin ratio was lower in E. coli mice at t=48 and higher at t=72 hours compared to controls. Conclusion This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU-AW. This article is protected by copyright. All rights reserved.

BIOSEB Instruments Used:
Grip strength test (BIO-GS3)