The novel compound 2--2-methyl-4-oxoquinazolin-3-4H--yl--N-p-tolylacetamide -P-TOL- -T-02- attenuates inflammatory and noci-ceptive transmission in experimental animal models of pain and inflammation

Authors
A. Masood, D. Chandrashekhar

Lab
Shri Neminath Jain Bramhacharyashram's Shriman Sureshdada Jain college of pharmacy Jain gurukul, Chandwad, India

Journal
International Journal of Scientific & Engineering Research

Abstract
Quinazoline-4(3H)-ones and its derivatives are versatile nitrogen heterocyclic compounds which have long been known as a promising class of biologically active compounds. Here we have investigated anti-inflammatory and anti-nociceptive potentials of some of the newly synthesized Quinazolines derivatives. Pharmacological activity was evaluated in the animal models of inflammation (Carrageenan induced paw edema, CFA induced inflammatory pain model) and Pain (formalin induced nociception) in male Sprague Dawley rats. We have evaluated T-02 compound and showed notably effective in reducing the not only carrageenan induced paw edema and CFA induced inflammatory pain but also formalin induced nociception. In carrageenan induced paw edema, CFA induced inflammatory pain and formalin induced nociception. W hen given orally, at similar doses produced significant reduction in paw volume, mechanical hyperalgesia (PWT -paw withdrawal threshold) and inhibition of nocifensive behavior (duration of paw licking and bitting) induced by subplanatar formalin injection. T-02 showed most prominent activity in both the animal models. T-02 at 1 mg/kg, 3 mg/kg, and 10 mg/kg significantly lowered nocifensive score in rats. The paw licking behavior in formalin test was more potently suppressed during the late phase (20–40 min, inflammatory) than in early phase (0–5 min, neurogenic) for T-02. Also; in CFA induced inflammatory pain model (T-02) at a dose of 1, 3 and 10 mg/kg significantly reduced the mechanical hyperalgesia (PW T -paw withdrawal threshold) show signs of anti- hyperalgesic activity and in carrageenan induce paw edema model 3 mg/kg, and 10 mg/kg (T-02) significantly reduced the edema reveal anti-inflammatory activity.

BIOSEB Instruments Used:
Von Frey Filaments (Bio-VF-M)