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Test de suspension caudale automatique- Sans-fil
Référence : BIO-TST5
Catégorie : Anxiété & Dépression
La version 5 du Test de Suspension Caudale de Bioseb, basée sur des capteurs de force ainsi que sur de l'acquisition vidéo, est la seule et unique solution totalement automatisée au monde pour effectuer un test de désespoir comportemental simple et objectif sur souris. Il s'agit d'une méthode rapide, fiable et sans douleur pour tester les effets psychotropes de drogues et substances comme les antidépresseurs et les sédatifs. Ce test classique et indolore pour la recherche sur l'anxiété et la dépression est désormais présenté avec un nouveau logiciel puissant et moderne, dans une version sans-fil BlueTooth.
Complètement automatique
Méthode 'scotch', indolore
Cages de suspension caudale de Bioseb
Présentation
Le test de suspension caudale a été développé en tant qu'alternative au test de nage forcée de Porsolt, et repose sur un concept similaire. Des souris, suspendue par la queue en utilisant un morceau de ruban adhésif (une méthode sans douleur), essaient, de manière innée, de s'échapper de cette situation non-naturelle. A force d'échouer dans leur tentative d'échappement, elles génèrent un état de désespoir qui se traduit par de l'immobilité. La magnitude de cette immobilité est considérée comme corrélée à un état dépressif, et est réduit de façon significative par les antidépresseurs. Le Test de Suspension Caudale a été décrit dans les années 80 par Steru et al. et permet l'évaluation rapide des effets psychotropes de drogues et substances (antidépresseurs, sédatifs), ce qui en fait un test classique et indolore pour la recherche sur l'anxiété et la dépression.
Le TST 4 de Bioseb est le seule test complètement automatique au monde! Il est basé sur le dessin original, le 'ITEMATIC-TST' décrit par Steru en 1987, et utilise à la fois des capteurs de force et un enregistrement vidéo. Le test ne génère aucune douleur sur l'animal: la queue de la souris est simplement fixée au capteur en utilisant un morceau de ruban adhésif.
Le dessin original ITEMATIC a été modifié, adapté et amélioré pour la compagnie Porsolt and Partners Pharmacology en 2000. L'instrument peut accommoder 3 souris dans 3 compartiments différents, et propose une hauteur au sol réglable, ainsi que des plaques anti-escalade pour les souris de lignée C57.
Le test de suspension caudale de Bioseb est un instrument offrant des fonctionnalités uniques, telles que la randomisation de la liste d'animaux, la mesure de 6 animaux simultanément, et le calcul direct des paramètres d'immobilité, d'énergie et de puissance en mouvement. L'utilisateur peut modifier les réglages pour s'adapter à son protocole de recherche. La valeur d'énergie est une méthode unique permettant de différentier le balancement passif et la lutte active. La puissance en mouvement est un calcul discriminant supplémentaire donnant une indication de la force de la souris.
Comme ça marche?
Fixation sans douleur
Le principe de mesure est basé sur la quantité de mouvement générée par une souris essayant d'échapper à sa suspension. Durant le test de suspension caudale, les mouvements des souris sont analysés en terme d'activité (active/non-active), d'énergie et de puissance développée dans le temps. Dans la mesure où l'observation humaine est fastidieuse, peu répétable, et prend du temps, le test de suspension caudale de Bioseb (TST) automatise l'intégralité de l'expérience.
L'opérateur prépare ses morceaux de ruban adhésif pour suspendre les souris et les cages contenant les animaux à tester. Une fois que l'expérience a été configurée via le logiciel et que la liste des animaux a été randomisée, l'opérateur suspend chaque souris individuellement en suivant la randomisation affichée à l'écran. Chaque voie est ensuite démarrée de manière individuelle en pressant une touche dédiée, afin de s'assurer que chaque animal est suspendu pour la même durée (ce qui est impossible avec un système basé uniquement sur la vidéo).
Lorsque le cycle expérimental est terminé, les souris sont retirés et le système demande à l'opérateur de suspendre le prochain groupe d'animaux. Après l'expérience, la totalité des groupes peut-être analysée, et des statistiques sur les minimums et maximums, moyennes, ainsi que sur les déviations standards, sont effectués en quelques secondes. Les données sont présentées de manière globale par groupe et détaillées pour chaque groupe par individus (souris).
Le logiciel TST-SOFT génère également des graphiques qui peuvent être exportées au format BMP pour animer de futures présentations scientifiques.
Fourni avec
• Un ou deux ensembles de 3 cages avec alimentation et câble USB nécessaire.
• 1 licence logicielle (dongle USB avec tag d'identification du produit)
• Caméra USB en option, avec tripode et câble USB
BIO-TST5
Fiche technique
- Dimensions globales
- 50 x 15 x 30 cm par jeu de 3 cages
- Matériaux
- PVC Noir & Blanc
- Nombre d'animaux
- 1-3 souris avec un jeu de cages 1-6 souris avec 2 jeux de cages
- Alimentation
- 110-230 Volts - 9 volts DC
- Fréquence d'échantillonnage
- 200 Hz pour les capteurs 15 images/seconde pour la caméra
- Applications
- Douleur
Métabolisme
Neurodégénérescence
Système Nerveux Central (SNC)
Système musculaire
Thématiques transversales
Troubles de l'humeur
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[title] => Na V 1-2 haploinsufficiency in Scn2a knock-out mice causes an autistic-like phenotype attenuated with age
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[title] => Depression_like symptoms of withdrawal in a genetic mouse model of binge methamphetamine intake
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[title] => Chlorogenic Acid from Hawthorn Berry -Crataegus pinnatifida Fruit- Prevents Stress Hormone_Induced Depressive Behavior- Through Monoamine Oxidase B_reactive Oxygen Species Signaling in Hippocampal Astrocytes of Mice
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[title] => Treatment with HC-070- a potent inhibitor of TRPC4 and TRPC5- leads to anxiolytic and antidepressant effects in mice
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[title] => Impaired microglia fractalkine signaling affects stress reaction and coping style in mice
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[image_presente] =>
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[title] => S 47445 Produces Antidepressant- and Anxiolytic Like Effects through Neurogenesis Dependent and Independent Mechanisms
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[image_presente] =>
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[title] => FGF-2 deficiency causes dysregulation of Arhgef6 and downstream targets in the cerebral cortex accompanied by altered neurite outgrowth and dendritic spine morphology
[paragraph_crop] => FGF-2 deficiency causes dysregulation of Arhgef6 and downstream targets in the cerebral cortex [...]
[image_presente] =>
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[url] => https://bioseb.com/fr/news/probable-involvement-of-p11-with-interferon-alpha-induced-depression-n819
[title] => Probable involvement of p11 with interferon alpha induced depression
[paragraph_crop] => Probable involvement of p11 with interferon alpha induced depression
[image_presente] =>
[date] => 2016-01-29 00:00:00
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[title] => Enriched environment decreases microglia and brain macrophages inflammatory phenotypes through adiponectin-dependent mechanisms- Relevance to depressive-like behavior
[paragraph_crop] => Enriched environment decreases microglia and brain macrophages inflammatory phenotypes through [...]
[image_presente] =>
[date] => 2015-07-22 00:00:00
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[title] => Synthesis and Evaluation of New 1-5-Diaryl-3-[4--methyl-sulfonyl-phenyl]-4-5-dihydro-1H-pyrazole Derivatives as Potential Antidepressant Agents
[paragraph_crop] => Synthesis and Evaluation of New [...]
[image_presente] =>
[date] => 2015-02-04 00:00:00
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[url] => https://bioseb.com/fr/news/antinociceptive-and-antidepressant-like-action-of-endomorphin-2-analogs-with-proline-surrogates-in-position-2-n641
[title] => Antinociceptive and antidepressant-like action of endomorphin-2 analogs with proline surrogates in position 2
[paragraph_crop] => Antinociceptive and antidepressant-like action of endomorphin-2 analogs with proline [...]
[image_presente] =>
[date] => 2014-09-01 00:00:00
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[url] => https://bioseb.com/fr/news/altered-skeletal-muscle-mitochondrial-biogenesis-but-improved-endurance-capacity-in-trained-opa1-deficient-mice-n516
[title] => Altered skeletal muscle mitochondrial biogenesis but improved endurance capacity in trained OPA1-deficient mice
[paragraph_crop] => Altered skeletal muscle mitochondrial biogenesis but improved endurance capacity in trained [...]
[image_presente] =>
[date] => 2013-12-01 00:00:00
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[title] => Génétique
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[title] => Métabolisme
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[title] => Phénotypage
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[id] => 514
[url] => https://bioseb.com/fr/news/early-postnatal-motor-experience-shapes-the-motor-properties-of-c57bl-and-6j-adult-mice--n514
[title] => Early postnatal motor experience shapes the motor properties of C57BL and 6J adult mice-
[paragraph_crop] => Early postnatal motor experience shapes the motor properties of C57BL/6J adult mice.
[image_presente] =>
[date] => 2013-11-30 00:00:00
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[id] => 513
[url] => https://bioseb.com/fr/news/antidepressant-and-anxiolytic-potential-of-the-multimodal-antidepressant-vortioxetine--lu-aa21004--assessed-by-behavioural-and-neurogenesis-outcomes-in-mice-n513
[title] => Antidepressant and anxiolytic potential of the multimodal antidepressant vortioxetine -Lu AA21004- assessed by behavioural and neurogenesis outcomes in mice
[paragraph_crop] => Antidepressant and anxiolytic potential of the multimodal antidepressant vortioxetine (Lu [...]
[image_presente] =>
[date] => 2013-10-30 00:00:00
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[title] => Publications
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[url] => https://bioseb.com/fr/news/neurochemical-changes-in-the-mouse-hippocampus-underlying-the-antidepressant-effect-of-genetic-deletion-of-p2x7-receptors-n515
[title] => Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors
[paragraph_crop] => Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic [...]
[image_presente] =>
[date] => 2013-06-21 00:00:00
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[title] => Publications
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[517] => Array
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[url] => https://bioseb.com/fr/news/the-absence-of-p2x7-receptors--p2rx7--on-non-haematopoietic-cells-leads-to-selective-alteration-in-mood-related-behaviour-with-dysregulated-gene-expression-and-stress-reactivity-in-mice--n517
[title] => The absence of P2X7 receptors -P2rx7- on non-haematopoietic cells leads to selective alteration in mood-related behaviour with dysregulated gene expression and stress reactivity in mice-
[paragraph_crop] => The absence of P2X7 receptors (P2rx7) on non-haematopoietic cells leads to selective [...]
[image_presente] =>
[date] => 2013-02-01 00:00:00
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[title] => Publications
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[434] => Array
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[url] => https://bioseb.com/fr/news/both-chronic-treatments-by-epothilone-d-and-fluoxetine-increase-the-short-term-memory-and-differentially-alter-the-mood-status-of-stop-and-map6-ko-mice-n434
[title] => Both chronic treatments by epothilone D and fluoxetine increase the short-term memory and differentially alter the mood status of STOP and MAP6 KO mice
[paragraph_crop] => Both chronic treatments by epothilone D and fluoxetine increase the short-term memory and [...]
[image_presente] =>
[date] => 2012-10-25 00:00:00
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[url] => https://bioseb.com/fr/news/anti-depressant-like-effect-of-vitexin-in-balb-and-c-mice-and-evidence-for-the-involvement-of-monoaminergic-mechanisms-n452
[title] => Anti-depressant-like effect of vitexin in BALB and c mice and evidence for the involvement of monoaminergic mechanisms
[paragraph_crop] => Anti-depressant-like effect of vitexin in BALB/c mice and evidence for the involvement of [...]
[image_presente] =>
[date] => 2012-10-22 00:00:00
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[id] => 430
[url] => https://bioseb.com/fr/news/differential-effects-of-early-environmental-enrichment-on-emotionality-related-behaviours-in-huntingtons-disease-transgenic-mice-n430
[title] => Differential effects of early environmental enrichment on emotionality-related behaviours in Huntington's disease transgenic mice
[paragraph_crop] => Differential effects of early environmental enrichment on emotionality-related behaviours in [...]
[image_presente] =>
[date] => 2012-10-02 00:00:00
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[id] => 398
[url] => https://bioseb.com/fr/news/the-deletion-of-stop-and-map6-protein-in-mice-triggers-highly-altered-mood-and-impaired-cognitive-performances---n398
[title] => The deletion of STOP and MAP6 protein in mice triggers highly altered mood and impaired cognitive performances-
[paragraph_crop] => The deletion of STOP/MAP6 protein in mice triggers highly altered mood and impaired cognitive [...]
[image_presente] =>
[date] => 2012-04-30 00:00:00
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[400] => Array
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[id] => 400
[url] => https://bioseb.com/fr/news/a-functional-tph2-c1473g-polymorphism-causes-an-anxiety-phenotype-via-compensatory-changes-in-the-serotonergic-system---n400
[title] => A Functional Tph2 C1473G Polymorphism Causes an Anxiety Phenotype via Compensatory Changes in the Serotonergic System-
[paragraph_crop] => A Functional Tph2 C1473G Polymorphism Causes an Anxiety Phenotype via Compensatory Changes in [...]
[image_presente] =>
[date] => 2012-04-11 00:00:00
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[title] => Phénotypage
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[id_prestablog_categorie] => 2
[title] => Publications
[link_rewrite] => publications
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[id] => 393
[url] => https://bioseb.com/fr/news/significantly-differential-diffusion-of-neuropathological-aggregates-in-the-brain-of-transgenic-mice-carrying-n-terminal-mutant-huntingtin-fused-with-green-fluorescent-protein--n393
[title] => Significantly differential diffusion of neuropathological aggregates in the brain of transgenic mice carrying N-terminal mutant huntingtin fused with green fluorescent protein-
[paragraph_crop] => Significantly differential diffusion of neuropathological aggregates in the brain of [...]
[image_presente] =>
[date] => 2012-03-16 00:00:00
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[title] => Dépression
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[title] => Fonction cardiaque
[link_rewrite] => Fonction-cardiaque
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[title] => Maladie de Huntington
[link_rewrite] => maladie-de-huntington
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[title] => Neurodégénérescence
[link_rewrite] => Neurodegenerescence
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[2] => Array
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[title] => Publications
[link_rewrite] => publications
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[15] => Array
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[id] => 389
[url] => https://bioseb.com/fr/news/pramipexole-is-active-in-depression-tests-and-modulates-monoaminergic-transmission--but-not-brain-levels-of-bdnf-in-mice---n389
[title] => Pramipexole is active in depression tests and modulates monoaminergic transmission- but not brain levels of BDNF in mice-
[paragraph_crop] => Pramipexole is active in depression tests and modulates monoaminergic transmission, but not [...]
[image_presente] =>
[date] => 2012-02-29 00:00:00
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[title] => Maladie de Parkinson
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[id_prestablog_categorie] => 12
[title] => Neurodégénérescence
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[title] => Publications
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[link_rewrite] => Troubles-de-l-humeur
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[id] => 371
[url] => https://bioseb.com/fr/news/increased-expression-of-the-vesicular-glutamate-transporter-1--vglut1--in-the-prefrontal-cortex-correlates-with-differential-vulnerability-to-chronic-stress-in-various-mouse-strains--effects-of-fluoxetine-and-mk-801---n371
[title] => Increased expression of the Vesicular Glutamate Transporter-1 -VGLUT1- in the prefrontal cortex correlates with differential vulnerability to chronic stress in various mouse strains- Effects of fluoxetine and MK-801-
[paragraph_crop] => Increased expression of the Vesicular Glutamate Transporter-1 (VGLUT1) in the prefrontal [...]
[image_presente] =>
[date] => 2012-01-30 00:00:00
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[title] => Publications
[link_rewrite] => publications
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[id] => 363
[url] => https://bioseb.com/fr/news/t-bet-deficient-mice-exhibit-resistance-to-stress-induced-development-of-depression-like-behaviors---n363
[title] => T-bet deficient mice exhibit resistance to stress-induced development of depression-like behaviors-
[paragraph_crop] => T-bet deficient mice exhibit resistance to stress-induced development of depression-like behaviors.
[image_presente] =>
[date] => 2011-12-15 00:00:00
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[title] => Publications
[link_rewrite] => publications
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[link_rewrite] => Troubles-de-l-humeur
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[356] => Array
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[id] => 356
[url] => https://bioseb.com/fr/news/severe-serotonin-depletion-after-conditional-deletion-of-the-vesicular-monoamine-transporter-2-gene-in-serotonin-neurons--neural-and-behavioral-consequences---n356
[title] => Severe Serotonin Depletion after Conditional Deletion of the Vesicular Monoamine Transporter 2 Gene in Serotonin Neurons- Neural and Behavioral Consequences-
[paragraph_crop] => Severe Serotonin Depletion after Conditional Deletion of the Vesicular Monoamine Transporter 2 [...]
[image_presente] =>
[date] => 2011-11-03 00:00:00
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[title] => Publications
[link_rewrite] => publications
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[title] => Troubles de l'humeur
[link_rewrite] => Troubles-de-l-humeur
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[id] => 288
[url] => https://bioseb.com/fr/news/the-deletion-of-the-microtubule-associated-stop-protein-affects-the-serotonergic-mouse-brain-network---n288
[title] => The deletion of the microtubule-associated STOP protein affects the serotonergic mouse brain network-
[paragraph_crop] => The deletion of the microtubule-associated STOP protein affects the serotonergic mouse brain [...]
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[title] => Behavioural and neuroplastic effects of the new-generation antidepressant agomelatine compared to fluoxetine in glucocorticoid receptor-impaired mice-
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[title] => Antidepressant-like effect of the methanolic extract from Bupleurum falcatum in the tail suspension test-
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[title] => Altered CB1 receptor and endocannabinoid levels precede motor symptom onset in a transgenic mouse model of Huntington's disease-
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[title] => Differential long-term effects of MDMA on the serotoninergic system and hippocampal cell proliferation in 5-HTT knock-out vs- wild-type mice-
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[url] => https://bioseb.com/fr/news/suitability-of-tamoxifen-induced-mutagenesis-for-behavioral-phenotyping--n160
[title] => Suitability of tamoxifen-induced mutagenesis for behavioral phenotyping-
[paragraph_crop] => Suitability of tamoxifen-induced mutagenesis for behavioral phenotyping.
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[url] => https://bioseb.com/fr/news/lasting-syndrome-of-depression-produced-by-reduction-in-serotonin-uptake-during-postnatal-development--evidence-from-sleep--stress--and-behavior--n155
[title] => Lasting Syndrome of Depression Produced by Reduction in Serotonin Uptake during Postnatal Development- Evidence from Sleep- Stress- and Behavior-
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[url] => https://bioseb.com/fr/news/calcineurin--protein-phosphatase-2b--is-involved-in-the-mechanisms-of-action-of-antidepressants--n102
[title] => Calcineurin -protein phosphatase 2B- is involved in the mechanisms of action of antidepressants-
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[url] => https://bioseb.com/fr/news/il-6-knockout-mice-exhibit-resistance-to-stress-induced-development-of-depression-like-behaviors--n84
[title] => IL-6 knockout mice exhibit resistance to stress-induced development of depression-like behaviors-
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[url] => https://bioseb.com/fr/news/early-life-blockade-of-5-hydroxytryptamine-1a-receptors-normalizes-sleep-and-depression-like-behavior-in-adult-knock-out-mice-lacking-the-serotonin-transporter--n78
[title] => Early Life Blockade of 5-Hydroxytryptamine 1A Receptors Normalizes Sleep and Depression-Like Behavior in Adult Knock-Out Mice Lacking the Serotonin Transporter-
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[title] => The tail suspension test as a model for assessing antidepressant activity- Review of pharmacological and genetic studies in mice-
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[url] => https://bioseb.com/fr/news/stress-induced-anhedonia-in-mice-is-associated-with-deficits-in-forced-swimming-and-exploration--n24
[title] => Stress-Induced Anhedonia in Mice is Associated with Deficits in Forced Swimming and Exploration-
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[title] => Genetic and Pharmacological Evidence of a Role for GABAB Receptors in the Modulation of Anxiety- and Antidepressant-Like Behavior-
[paragraph_crop] => Genetic and Pharmacological Evidence of a Role for GABAB Receptors in the Modulation of [...]
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