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Les Kits contextuels pour T2CT ont été conçus pour enrichir les études de préférence de place...
Douleur
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Distribution Pondérale Dynamique 2.0
Référence : BIO-DWB-DUAL
Catégorie : Douleur - Spontanée - Déficit de Posture
Un système permettant l'analyse de la posture des animaux par la répartition du poids sur chacune des 4 pattes. Le DWB est un test unique dans l'étude de nombreux modèles de douleurs, principalement non évoquée , neuropathique ou encore inflammatoire ainsi que pour les études sur l'osthéoarthrite et divers affections du SNC.
Le nouvelle version DWB2 intègre une nouvelle interface logiciel plus simple avec gestion des listes d'animaux ainsi que de nouveaux algorithmes d'analyse automatique de la posture.
Historique
• 2006 - Test d'Incapacitance / Distribution Pondérale Statique: Premier test de mesure de la douleur spontanée utilisé en analgésie/nociception. L’appareil permet la mesure de la répartition du poids d’un rongeur en contention sur ses pattes arrières.
• 2008 - Test de Distribution Pondérale Dynamique Basique: L’instrument permet la mesure de la répartition du poids de l’animal sur ses 4 pattes et permet de s’affranchir de la contention.
• 2011 - Test de Distribution Pondérale Dynamique Avancé: Nouvelle version logiciel du DWB permettant le suivi vidéo de l’animal afin d’automatiser une partie du test.
• 2018 - Test de Distribution Pondérale Dynamique 2.0: Nouveau logiciel en 3 onglets avec performances d’automatisations améliorées et intégration de nouvelles fonctionnalités majeures.
Présentation
Distribution Pondérale Dynamique Avancée
Détail avec souris
Pour des raisons éthiques et afin d’étudier le niveau d’inconfort ou douleur non-évoquée des rongeurs, des tests permettant l’évaluation de la douleur sans provoquer de stimulus sur les animaux ont été développés. Ces tests dits « d’incapacitance » permettent la mesure de la répartition du poids sur les 2 pattes arrière des rongeurs en contention, impliquant un niveau de stress élevé pour les animaux.
Depuis son lancement en 2008, le test de distribution pondérale dynamique DWB est le seul test d’évaluation de la douleur non évoquée des rongeurs permettant de s’affranchir de cette contention des animaux. Il est aussi le seul instrument capable de mesurer avec précision l’évolution de la posture des rongeurs par la mesure de répartition du poids sur chacune de leurs 4 pattes.
Ainsi, le DWB permet de travailler avec des animaux libres de leurs déplacements dans une arène de dimensions adaptées aux rats ou à la souris. Cela permet de réduire considérablement le niveau de stress lié à la contention de l’animal et d’améliorer ainsi la fiabilité des résultats.
Validé par plus d’une trentaine de publications, cet instrument a permis de répondre à de nombreuses interrogations dans les modèles de douleurs non évoquées, neuropathiques, inflammatoires ou encore osthéoartritiques. Plus récemment, le cadre d’utilisation du DWB a dépassé les modèles de douleur et l’analyse de la posture s’est avéré idéale pour l’étude de troubles osseux (fractures, cancers, arthrose..), de différents problèmes du système nerveux central (ischémies) ou encore du système vestibulaire (vertige).
Principe de fonctionnement
L’animal est placé dans la cage du DWB (Dynamic Weight Bearing) sur une durée d’environ 5 minutes.
Durant le test, le DWB couple l’utilisation de deux technologies :
• Une matrice d’environ 2000 capteurs de force de grande précision (0.02g chez la souris et 0.15g chez la souris) : Cette matrice constitue le sol de la cage sur lequel l’animal évolue librement. Les capteurs permettent la mesure en grammes de la répartition du poids des animaux sur chacune de leurs 4 pattes. L’appareil utilise spécifiquement des capteurs pour rats ou pour souris avec un niveau de sensibilité adapté (de 0 à 4g chez la souris et de 0 à 40g chez le rats).
• Un suivi vidéo de l’animal : L’animal est filmé par-dessus grâce à une caméra haute résolution. La vidéo est analysée en temps réel lors du test par un logiciel de tracking permettant une analyse précise de la morphologie de l’animal.
Le logiciel est donc capable d’analyser précisément la position de l’animal durant l’ensemble du test afin d’identifier automatiquement la position des 4 pattes sur les capteurs de force.
L’acquisition des informations provenant des capteurs de force et de la caméra se faisant à haute fréquence (30 htz), le DWB est capable d’analyser les changements de positions rapides des animaux permettant des mesures complètes afin d’analyser précisément l’évolution de la posture des animaux immobiles ou en mouvements.
Versions et installation
Nous proposons un passage vers la nouvelle version pour les utilisateurs du DWB1. Votre matériel actuel est parfaitement compatible et seule une mise à jour du logiciel sera nécessaire.
Vos acquisitions faites avec le DWB1 pourront être analysées avec le nouveau DWB2 afin de bénéficier de l’interface et de la puissance d’analyse du nouveau logiciel.
Le DWB avancé est disponible en 3 versions: RAT (BIO-DWB-AUTO-R), SOURIS (BIO-DWB-AUTO-M), COMBO RAT-SOURIS (BIO-DWB-AUTO-DU).
Nouveau Système de Distribution Pondérale Semi-Automatisé pour Rat et Souris, par Bioseb: caméra, couvercle, cage et animal
BIO-DWB-DUAL
Fiche technique
- Résolution caméra
- Caméra USB 640x480 haute résolution fournie avec lentille, fixable sur le couvercle - une fois les réglages effectuées, inutile d'ajuster entre les animaux
- Applications
- Arthrite & Arthrose
Douleur
Système Nerveux Central (SNC)
Système musculaire
Système sensoriel
Thématiques transversales
Troubles de l'humeur
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[title] => Total Knee Arthroplasty with a Ti6Al4V and PEEK Prosthesis on an Osteoarthritis Rat Model- Behavioral and Neurophysiological Analysis
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[title] => Pain and knee damage in male and female mice in the medial meniscal transection-induced osteoarthritis Pain and knee damage in male and female mice in the medial meniscal transection-induced osteoarthritis
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[title] => Yokukansan Alleviates Cancer Pain by Suppressing Matrix Metalloproteinase-9 in a Mouse Bone Metastasis Model
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[title] => Narciclasine exerts anti-inflammatory actions by blocking leukocyteÐendothelial cell interactions and down-regulation of the endothelial TNF receptor 1
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[title] => Intra-articular treatment with triamcinolone acetonide-loaded liposomes in the rat high-fat diet groove model
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[title] => Intra-articular injection of triamcinolone acetonide releasing biomaterial microspheres inhibits pain and inflammation in an acute arthritis model
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[title] => Effects of Mollugo pentaphylla extract on monosodium urate crystal-induced gouty arthritis in mice
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[title] => Dynamic weight bearing analysis is effective for evaluation of tendinopathy using a customized corridor with multi-directional force sensors in a rat model
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[title] => Correlation between ?CT imaging- histology and functional capacity of the osteoarthritic knee in the rat model of osteoarthritis
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[title] => Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway
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[title] => A multi-target approach for pain treatment- dual inhibition of fatty acid amide hydrolase and TRPV1 in a rat model of osteoarthritis
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[title] => Evaluation of Dynamic Weight Bearing for Measuring Nonevoked Inflammatory Hyperalgesia in Mice
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[title] => Orthopedic surgery and bone fracture pain are both significantly attenuated by sustained blockade of nerve growth factor
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[title] => Polytherapy with a combination of three repurposed drugs -PXT3003- down-regulates Pmp22 over-expression and improves myelination- axonal and functional parameters in models of CMT1A neuropathy
[paragraph_crop] => Polytherapy with a combination of three repurposed drugs (PXT3003) down-regulates Pmp22 [...]
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[title] => NGF blockade at early times during bone cancer development attenuates bone destruction and increases limb use
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[title] => Use of dynamic weight bearing as a novel end-point for the assessment of abdominal pain in the LPS-induced peritonitis model in the rat
[paragraph_crop] => Use of dynamic weight bearing as a novel end-point for the assessment of abdominal pain in the [...]
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[title] => Repair of the injured spinal cord by implantation of a synthetic degradable block copolymer in rat
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[url] => https://bioseb.com/fr/news/pain-assessment-in-animal-models-of-osteoarthritis-n812
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[url] => https://bioseb.com/fr/news/a-new-method-to-assess-weight-bearing-distribution-after-central-nervous-system-lesions-in-rats-n497
[title] => A new method to assess weight-bearing distribution after central nervous system lesions in rats
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[image_presente] =>
[date] => 2013-11-04 00:00:00
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[title] => A new method to assess weight-bearing distribution after central nervous system lesions in rats
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[date] => 2013-10-26 00:00:00
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[url] => https://bioseb.com/fr/news/spinal-nts2-receptor-activation-reverses-signs-of-neuropathic-pain--n493
[title] => Spinal NTS2 receptor activation reverses signs of neuropathic pain-
[paragraph_crop] => Spinal NTS2 receptor activation reverses signs of neuropathic pain.
[image_presente] =>
[date] => 2013-06-12 00:00:00
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[title] => Comparison of evoked and non-evoked endpoints in a rodent pain model and potential relevance to non-evoked human pain-
[paragraph_crop] => Comparison of evoked and non-evoked endpoints in a rodent pain model and potential relevance [...]
[image_presente] =>
[date] => 2012-10-15 00:00:00
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[title] => A micro-imaging study linking bone cancer pain with tumor growth and bone resorption in a rat model
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[date] => 2012-09-30 00:00:00
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[url] => https://bioseb.com/fr/news/use-of-dynamic-weight-bearing-as-a-novel-end-point-for-the-assessment-of-freunds-complete-adjuvant-induced-hypersensitivity-in-mice-n480
[title] => Use of dynamic weight bearing as a novel end-point for the assessment of Freund's Complete Adjuvant induced hypersensitivity in mice
[paragraph_crop] => Use of dynamic weight bearing as a novel end-point for the assessment of Freund's Complete [...]
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[url] => https://bioseb.com/fr/news/inflammation-induced-decrease-in-voluntary-wheel-running-in-mice--a-nonreflexive-test-for-evaluating-inflammatory-pain-and-analgesia--n396
[title] => Inflammation-induced decrease in voluntary wheel running in mice- a nonreflexive test for evaluating inflammatory pain and analgesia-
[paragraph_crop] => Inflammation-induced decrease in voluntary wheel running in mice: a nonreflexive test for [...]
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[id] => 375
[url] => https://bioseb.com/fr/news/neuroplasticity-of-sensory-and-sympathetic-nerve-fibers-in-the-painful-arthritic-joint---n375
[title] => Neuroplasticity of sensory and sympathetic nerve fibers in the painful arthritic joint-
[paragraph_crop] => Neuroplasticity of sensory and sympathetic nerve fibers in the painful arthritic joint.
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[date] => 2012-01-13 00:00:00
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[url] => https://bioseb.com/fr/news/weight-bearing-evaluation-in-inflammatory--neuropathic-and-cancer-chronic-pain-in-freely-moving-rats-n417
[title] => Weight bearing evaluation in inflammatory- neuropathic and cancer chronic pain in freely moving rats
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[image_presente] =>
[date] => 2011-09-01 00:00:00
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[title] => Thématiques transversales
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[416] => Array
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[url] => https://bioseb.com/fr/news/nav1-9-channel-contributes-to-mechanical-and-heat-pain-hypersensitivity-induced-by-subacute-and-chronic-inflammation-n416
[title] => Nav1-9 Channel Contributes to Mechanical and Heat Pain Hypersensitivity Induced by Subacute and Chronic Inflammation
[paragraph_crop] => Nav1.9 Channel Contributes to Mechanical and Heat Pain Hypersensitivity Induced by Subacute [...]
[image_presente] =>
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[url] => https://bioseb.com/fr/news/acid-sensing-ion-channels-in-postoperative-pain---n316
[title] => Acid-Sensing Ion Channels in Postoperative Pain-
[paragraph_crop] => Acid-Sensing Ion Channels in Postoperative Pain.
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[date] => 2011-04-20 00:00:00
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[id] => 283
[url] => https://bioseb.com/fr/news/behavioral--medical-imaging-and-histopathological-features-of-a-new-rat-model-of-bone-cancer-pain---n283
[title] => Behavioral- Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain-
[paragraph_crop] => Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain.
[image_presente] =>
[date] => 2010-10-29 00:00:00
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[title] => Cancer
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[title] => Fractures
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[title] => Publications
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[id_prestablog_categorie] => 22
[title] => Système squelettique
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[title] => Thématiques transversales
[link_rewrite] => Thematiques-transversales
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[278] => Array
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[id] => 278
[url] => https://bioseb.com/fr/news/alteration-of-sensory-neurons-and-spinal-response-to-an-experimental-osteoarthritis-pain-model---n278
[title] => Alteration of sensory neurons and spinal response to an experimental osteoarthritis pain model-
[paragraph_crop] => Alteration of sensory neurons and spinal response to an experimental osteoarthritis pain model.
[image_presente] =>
[date] => 2010-10-01 00:00:00
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[title] => Domaines de recherche divers
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[id_prestablog_categorie] => 2
[title] => Publications
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[id] => 276
[url] => https://bioseb.com/fr/news/articular-mesenchymal-stem-cell--msc--and-effects-of-growth-differentiation-factor-5--gdf-5---n276
[title] => Articular Mesenchymal Stem Cell -MSC- and Effects of Growth Differentiation Factor-5 -GDF-5--
[paragraph_crop] => Articular Mesenchymal Stem Cell (MSC) and Effects of Growth Differentiation Factor-5 (GDF-5).
[image_presente] =>
[date] => 2010-09-20 00:00:00
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[title] => Arthrite & Arthrose
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[title] => Articulations
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[id] => 165
[url] => https://bioseb.com/fr/news/polyamine-deficient-diet-to-relieve-pain-hypersensitivity--n165
[title] => Polyamine deficient diet to relieve pain hypersensitivity-
[paragraph_crop] => Polyamine deficient diet to relieve pain hypersensitivity.
[image_presente] =>
[date] => 2008-06-30 00:00:00
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[title] => Allodynie mécanique & Hyperalgésie
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1Nombre de publications:
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