Ginsenosides- salidroside- and syringin complex exhibits anti-fatigue in exhaustive exercise rats

Authors
Yung-Chun Wu, Yu Zhi Lian, Hongwei Zhao, Lei Wang, Deshan Ning, Jane C-J Chao


Lab

Journal
International Journal of Medical Sciences

Abstract
Intense or prolonged exercise could cause oxidative damage in muscles, and lipid peroxidation product MDA level could be correspondingly increased42, which was commonly used as an indicator of lipid peroxidation16. However, our study revealed that CHP intervention did not alter MDA levels in rat serum, liver, and gastrocnemius muscles. Increased MDA levels in tissues after exhaustive exercise still remained controversial because hepatic MDA level was not altered after acute exhaustive exercise compared to that after chronic exercise43. Our study also found that CHP intervention tended to increase serum SOD activity. Oral administration of salidroside (15-30 mg/kg bw) for 14 days prior to exhaustive swimming exercise increased nuclear factor erythroid 2-related factor 2 (Nrf2) as a transcription activator of antioxidant enzymes such as SOD in rat myocardial cells, which could lead to reduce oxidative stress and prevent myocardial damage. Additionally, Nrf2 protein expression in rat myocardial cells was positively associated with salidroside intervention concentration44. Thus, it is reasonable to assume that enhancing Nrf2 protein expression could be correlated to alleviate exercise-induced oxidative stress and further strengthen exercise performance, suggesting that CHP intervention containing salidroside might regulate antioxidative pathway to achieve anti-fatigue effect. The study for the correlation of CHP intervention and Nrf2 related protein expression is required to validate the possible mechanism. However, our study did not find the changes of SOD activity in liver and skeletal muscles among the four groups, even SOD activity in skeletal muscles tended to be increased after high-dose CHP intervention but still not significantly different. A pervious study revealed that SOD activity between serum and liver was weakly correlated (r= 0.21,p< 0.05), but not associated between serum and skeletal muscles (p> 0.05) in physiologically normal rats45, indicating that SOD activity could not be consistent between serum and tissues. Moreover, we cannot rule out the possible alteration in SOD activity in the certain isoenzyme in tissues after CHP intervention because we only measured the activity of total SOD. There are three SOD isoenzymes in mammals, Cu-Zn SOD (SOD1) in cytosol, Mn-SOD (SOD2) in mitochondrial matrix, and extracellular Cu-Zn SOD (SOD3) secreted from the tissue to the extracellular space46. Additionally, we speculate that SOD activity in serum or tissues could be altered differently before and after exhaustive exercise against oxidative stress, and changed earlier in serum compared to that in liver and skeletal muscles.

Keywords/Topics
Chinese herb; exercise performance; lactate clearance; antioxidation; anti-inflammation; rats

BIOSEB Instruments Used:
Grip strength test (BIO-GS4)

Source :

https://pmc.ncbi.nlm.nih.gov/articles/PMC11659827/

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Test d'agrippement

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