Inflammatory Pain Upregulates Spinal Inhibition via Endogenous Neurosteroid Production-

P. Poisbeau, C. Patte-Mensah, A. F. Keller, M. Barrot, J.-D. Breton et al.

Centre National de la Recherche Scientifique/Université Louis Pasteur, Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France.

The Journal of Neurosciences

Inhibitory synaptic transmission in the dorsal horn (DH) of the spinal cord plays an important role in the modulation of nociceptive messages because pharmacological blockade of spinal GABAA receptors leads to thermal and mechanical pain symptoms. Here, we show that during the development of thermal hyperalgesia and mechanical allodynia associated with inflammatory pain, synaptic inhibition mediated by GABAA receptors in lamina II of the DH was in fact markedly increased. This phenomenon was accompanied by an upregulation of the endogenous production of 5_-reduced neurosteroids, which, at the spinal level, led to a prolongation of GABAA receptor-mediated synaptic currents and to the appearance of a mixed GABA/glycine cotransmission. This increased inhibition was correlated with a selective limitation of the inflammation-induced thermal hyperalgesia, whereas mechanical allodynia remained unaffected. Our results show that peripheral inflammation activates an endogenous neurosteroid-based antinociceptive control, which discriminates between thermal and mechanical hyperalgesia.

BIOSEB Instruments Used:
Rodent pincher - analgesia meter (BIO-RP-M)

Publication request

Merci de votre intérêt pour notre gamme de produits et de votre demande pour cette publication qui vous sera envoyée si l'équipe de recherche et la revue le permettent. Notre équipe commerciale vous contactera dans les plus brefs délais.