A combination of two human monoclonal antibodies cures symptomatic rabies

Authors
GD de Melo, F Sonthonnax, G Lepousez et al

Lab
Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France

Journal
EMBO Molecular Medicine

Abstract
Rabies is a neglected disease caused by a neurotropic Lyssavirus, transmitted to humans predominantly by the bite of infected dogs. Rabies is preventable with vaccines or proper post_exposure prophylaxis (PEP), but it still causes about 60,000 deaths every year. No cure exists after the onset of clinical signs, and the case_fatality rate approaches 100% even with advanced supportive care. Here, we report that a combination of two potent neutralizing human monoclonal antibodies directed against the viral envelope glycoprotein cures symptomatic rabid mice. Treatment efficacy requires the concomitant administration of antibodies in the periphery and in the central nervous system through intracerebroventricular infusion. After such treatment, recovered mice presented good clinical condition, viral loads were undetectable, and the brain inflammatory profile was almost normal. Our findings provide the unprecedented proof of concept of an antibody_based therapeutic approach for symptomatic rabies.

BIOSEB Instruments Used:
Rotarod (BX-ROD),Aron Test or Four Plates Test (LE830)