Maladie d'Alzheimer - page 2 - Publications Scientifiques

Dernière publication 05/02/2010

Generation of conditional alleles for APP and APLP2-

Proteolytical cleavage of the beta-amyloid precursor protein (APP) generates beta-amyloid, which is deposited in the brains of patients suffering...

Array
(
    [id_prestablog_news] => 252
    [id_shop] => 1
    [date] => 2010-02-05 00:00:00
    [date_modification] => 2024-02-09 14:15:11
    [langues] => ["1","2"]
    [actif] => 1
    [slide] => 0
    [url_redirect] => 
    [average_rating] => 
    [number_rating] => 
    [author_id] => 1
    [featured] => 0
    [ishero] => 0
    [prim_key] => 502
    [id_lang] => 2
    [title] => Generation of conditional  alleles for APP and APLP2-
    [paragraph] => Generation of conditional  alleles for APP and APLP2.
    [content] => 
Authors
J.P. Mallm, J.A. Tschäpe, M. Hick, M.A. Filippov, U.C. Müller.

 Lab
University of Heidelberg, Institute of Pharmacy and Molecular Biotechnology, Department of Bioinformatics and Functional Genomics, Heidelberg, Germany.
Journal
Genesis
Abstract
Proteolytical cleavage of the beta-amyloid precursor protein (APP) generates beta-amyloid, which is deposited in the brains of patients suffering from Alzheimer's disease (AD). Despite the well-established key role of APP for AD pathogenesis, the physiological function of APP and its close homologues APLP1 and APLP2 remains poorly understood. Previously, we generated APP(-/-) mice that proved viable, whereas APP(-/-)APLP2(-/-) mice and triple knockouts died shortly after birth, likely due to deficits of neuromuscular synaptic transmission. Here, we generated conditional knockout alleles for both APP and APLP2 in which the promoter and exon1 were flanked by loxP sites. No differences in expression were detectable between wt and floxed alleles, whereas  alleles were obtained upon crossing with Cre-transgenic deleter mice. These mice will now allow for tissue and time-point controlled knockout of both genes.
BIOSEB Instruments Used
Grip strength test (BIO-GS3)

Keywords/Topics
Maladie d'Alzheimer; Neurodégénérescence; Infection
    [meta_description] => 
    [meta_keywords] => http://onlinelibrary.wiley.com/doi/10.1002/dvg.20601/abstract
    [meta_title] => 
    [link_rewrite] => generation-of-conditional--alleles-for-app-and-aplp2-
    [actif_langue] => 1
    [read] => 1073
    [count_comments] => 0
    [id] => 252
    [categories] => Array
        (
            [99] => Array
                (
                    [id_prestablog_categorie] => 99
                    [title] => Infection
                    [link_rewrite] => Infection
                )

            [46] => Array
                (
                    [id_prestablog_categorie] => 46
                    [title] => Maladie d'Alzheimer
                    [link_rewrite] => maladie-d-alzheimer
                )

            [12] => Array
                (
                    [id_prestablog_categorie] => 12
                    [title] => Neurodégénérescence
                    [link_rewrite] => Neurodegenerescence
                )

            [2] => Array
                (
                    [id_prestablog_categorie] => 2
                    [title] => Publications
                    [link_rewrite] => publications
                )

        )

    [authors] => 
    [paragraph_crop] => Generation of conditional  alleles for APP and APLP2.
    [link_for_unique] => 1
    [products_liaison] => Array
        (
            [48] => Array
                (
                    [name] => Test d'agrippement
                    [description_short] => 
Une méthode simple pour quantifier objectivement la force musculaire des rats et souris et l'effet de drogues, toxines, maladies musculaires (ex: myopathie) et neurodégénératives. Cette mesure de force est souvent employée en association avec le test de coordination motrice ROTAROD: un sujet présentant une coordination normale montrera des résultats médiocres en cas de faible force musculaire. Un must pour vos recherches sur l'activité, la coordination et le contrôle musculaire: particulièrement utile pour vos études sur les maladies de Parkinson et Huntington.
Nouveautés GS4 - 2023: Écran couleur rétroéclairé (meilleure lisibilité), pédale de remise à zéro, durée de batterie optimisée, taille de mémoire augmentée à 500 valeurs, compteur d'animaux, port USB (transfert de données/charge)

                    [thumb] => 
                    [img_empty] => /var/www/vhosts/de3310.ispfr.net/bioseb2024/modules/prestablog/views/img/product_link_white.jpg
                    [image_presente] => 1
                    [link] => https://bioseb.com/fr/activite-systeme-moteur-coordination/48-grip-strength-test.html
                )

        )

)
1