Activity, Motor Control & Coordination
Pain - Thermal Allodynia / Hyperalgesia
Pain - Spontaneous Pain - Postural Deficit
Pain - Mechanical Allodynia / Hyperalgesia
Anxiety & Depression Disorder
Learning/Memory - Attention - Addiction
Physiology & Respiratory Research
Metabolism - Food & Drink Intake
Pain
Central Nervous System (CNS)
Neurodegeneration
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Motor control
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Cross-disciplinary subjects
Preclinical studies and opioids: role in crisis management in the United States Authors
Wu X, Wu C, Zhou T.
Lab
Chinese Academy of Sciences, Shenzhen China
Journal
Brain Behav.
Abstract
Objectives N6‐methyladenosine (m6A) plays a crucial role in regulating neuroplasticity and different brain functions at the posttranscriptional level. However, it remains unknown whether m6A modification is involved in acute and chronic morphine exposure. Materials and methods In this study, we conducted a direct comparison of m6A levels and mRNA expression of m6A‐associated factors between morphine‐treated and nontreated C57BL/6 wild‐type mice. We established animal models of both acute and chronic morphine treatment and confirmed the rewarding effects of chronic morphine treatment using the conditioned place preference (CPP) assay. The activation status of different brain regions in response to morphine was assessed by c‐fos staining. To assess overall m6A modification levels, we employed the m6A dot blot assay, while mRNA levels of m6A‐associated proteins were measured using a quantitative polymerase chain reaction (qPCR) assay. These analyses were performed to investigate whether and how m6A modification and m6A‐associated protein expression will change following morphine exposure. Results The overall m6A methylation and mRNA levels of m6A‐associated proteins were not significantly altered in brain regions that were either activated or not activated during acute morphine stimulation. Similarly, the overall m6A modification and mRNA levels of m6A‐associated proteins remained unaffected in several key brain regions associated with reward following chronic morphine exposure. Conclusion This study showed that the overall m6A modification level and mRNA expression levels of m6A‐associated factors were not affected after acute and chronic morphine exposure in different brain regions, indicating m6A modification may not be involved in brain response to morphine exposure.
BIOSEB Instruments Used:
Cold Hot Plate Test (BIO-CHP)