Douleurs neuropathiques - page 6 - Publications Scientifiques

Dernière publication 17/05/2018

Hydrogen-Rich Saline Activated Autophagy via HIF-1_ Pathways in Neuropathic Pain

Neuropathic pain is a chronic and intractable pain, with very few effective analgesics. It involves an impaired cell autophagy process....

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    [title] => Hydrogen-Rich Saline Activated Autophagy via HIF-1_ Pathways in Neuropathic Pain
    [paragraph] => Hydrogen-Rich Saline Activated Autophagy via HIF-1_ Pathways in Neuropathic Pain Model
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Authors
H Wang, X Huo, H Chen, B Li, J Liu, W Ma, X Wang, K Xie, Y Yu,K Shi

 Lab
Pain Management Center, Second Hospital of Tianjin Medical University, and Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin Research Institute of Anesthesiology Tianjin, China
Journal
BioMed Research International
Abstract
Neuropathic pain is a chronic and intractable pain, with very few effective analgesics. It involves an impaired cell autophagy process. Hydrogen-rich saline (HRS) reportedly reduces allodynia and hyperalgesia in a neuropathic pain model; however, it is unknown whether these effects involve autophagy induction.Methods. We investigated the relationship between HRS and cell autophagy in a neuropathic pain model generated by chronic constriction injury (CCI) in Sprague–Dawley rats. Rats received an intraperitoneal injection of HRS (10_mL/kg daily, from 1 day before until 14 days after CCI), 3MA (autophagy inhibitor), 2ME2 (HIF-1_ inhibitor), or EDHB (HIF-1_ agonist). The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were tested 1 day before and 1, 3, 7, 10, and 14 days after the operation. HIF-1_ and cell autophagy markers in the spinal cord were evaluated by western blotting and real-time PCR assays at 14 days after CCI. Autophagosomes with double membranes were identified by transmission electron microscopy. Results. CCI caused behavioral hypersensitivity to mechanical and thermal stimulation in the hind-paw of the injured side. HRS improved MWT and TWL, activated autophagy, and increased autophagosomes and autolysosomes in CCI rats. 3-MA aggravated hyperalgesia and allodynia and suppressed autophagy, while EDHB attenuated hyperalgesia and activated the autophagy procedure and the HIF-1_downstream target gene BNIP3. HIF-1_ inhibitors reversed the regulatory effects of HRS on autophagy in CCI rats at 14 days after spinal cord injury. Conclusion. HRS reduced mechanical hyperalgesia and activation of cell autophagy in neuropathic pain through a HIF1-dependent pathway.
BIOSEB Instruments Used
Electronic Von Frey 4 (BIO-EVF4),Electronic Von Frey 5 with embedded camera (BIO-EVF5)

Keywords/Topics
Douleurs neuropathiques; Douleur
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Une solution rapide pour déterminer le seuil de sensibilité à la douleur mécanique chez les rongeurs (rats et souris). Maintenant en version sans-fil, pour être libéré des câbles!

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L'EVF5 intègre une caméra embarquée au niveau de la zone de stimulation et fonctionne avec un nouveau logiciel dédié révolutionnaire

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