Phénotypage - page 3 - Publications Scientifiques

Dernière publication 15/08/2008

Reliability- robustness- and reproducibility in mouse behavioral phenotyping- a

Establishing standard operating procedures (SOPs) as tools for the analysis of behavioral phenotypes is fundamental to mouse functional genomics....

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    [title] => Reliability- robustness- and reproducibility in mouse behavioral phenotyping- a 
    [paragraph] => Reliability, robustness, and reproducibility in mouse behavioral phenotyping: a cross-laboratory study.
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Authors
S. Mandillo, V. Tucci, S.M. Hölter, H. Meziane, M. Al Banchaabouchi.

 Lab
Institute of Developmental Genetics, Gesellschaft für Strahlenforschung-National Research Center for Environment and Health, Neuherberg, Germany.
Journal
Physiological genomics
Abstract
Establishing standard operating procedures (SOPs) as tools for the analysis of behavioral phenotypes is fundamental to mouse functional genomics. It is essential that the tests designed provide reliable measures of the process under investigation but most importantly that these are reproducible across both time and laboratories. For this reason, we devised and tested a set of SOPs to investigate mouse behavior. Five research centers were involved across France, Germany, Italy, and the UK in this study, as part of the EUMORPHIA program. All the procedures underwent a cross-validation experimental study to investigate the robustness of the designed protocols. Four inbred reference strains (C57BL/6J, C3HeB/FeJ, BALB/cByJ, 129S2/SvPas), reflecting their use as common background strains in mutagenesis programs, were analyzed to validate these tests. We demonstrate that the operating procedures employed, which includes open field, SHIRPA, grip-strength, rotarod, Y-maze, prepulse inhibition of acoustic startle response, and tail flick tests, generated reproducible results between laboratories for a number of the test output parameters. However, we also identified several uncontrolled variables that constitute confounding factors in behavioral phenotyping. The EUMORPHIA SOPs described here are an important start-point for the ongoing development of increasingly robust phenotyping platforms and their application in large-scale, multicentre mouse phenotyping programs.
BIOSEB Instruments Used
Grip strength test (BIO-GS3)

Keywords/Topics
Phénotypage; Thématiques transversales
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Une méthode simple pour quantifier objectivement la force musculaire des rats et souris et l'effet de drogues, toxines, maladies musculaires (ex: myopathie) et neurodégénératives. Cette mesure de force est souvent employée en association avec le test de coordination motrice ROTAROD: un sujet présentant une coordination normale montrera des résultats médiocres en cas de faible force musculaire. Un must pour vos recherches sur l'activité, la coordination et le contrôle musculaire: particulièrement utile pour vos études sur les maladies de Parkinson et Huntington.
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