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Dernière publication 24/04/2022

Serpine1 Regulates Peripheral Neutrophil Recruitment and Acts as Potential Targe

Introduction: Peripheral neutrophil infiltration can exacerbate ischemiaÐreperfusion injury. We focused on the relationship between various...

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    [title] => Serpine1 Regulates Peripheral Neutrophil Recruitment and Acts as Potential Targe
    [paragraph] => Serpine1 Regulates Peripheral Neutrophil Recruitment and Acts as Potential Target in Ischemic Stroke
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Authors
Z Pu, X Bao, S Xia et al

 Lab
Nanjing University, Nanjing, Jiangsu, PeopleÕs Republic of China
Journal
Journal of Inflammation Research
Abstract
Introduction: Peripheral neutrophil infiltration can exacerbate ischemiaÐreperfusion injury. We focused on the relationship between various peripheral immune cells and cerebral ischemiaÐreperfusion (I/R) injury.
Methods: In this study, we investigated the effects of dauricine on neuronal injury induced by ischemiaÐreperfusion and peripheral immune cells after ischemic stroke in mouse model, and we explored the undefined mechanisms of regulating peripheral immune cells through RNA sequencing and various biochemical verification in vitro and in vivo.
Results: We found that dauricine improved the neurological deficits of I/R injury, reduced the infarct volume, and improved the neurological scores. Furthermore, dauricine reduced the infiltration of neutrophils into the brain after MCAO-R and increased peripheral neutrophils but unchanged the permeability of the endotheliocyte Transwell system in an in vitro blood-brain barrier (BBB) model. RNA sequencing showed that chemotaxis factors, such as CXCL3, CXCL11, CCL20, CCL22, IL12a, IL23a, and serpine1, might play a crucial role. Overexpression of serpine1 reversed LPS-induced migration of neutrophils. Dauricine can directly bind with serpine1 in ligandÐreceptor docking performed with the Autodock and analyzed with PyMOL.
Conclusion: We identified chemotaxis factor serpine1 played a crucial role in peripheral neutrophil infiltration, which may contribute to reduce the neuronal injury induced by ischemiaÐreperfusion. These findings reveal that serpine1 may act as a potential treatment target in the acute stage of ischemic stroke.
BIOSEB Instruments Used
Grip strength test (BIO-GS3)

Keywords/Topics
Accidents ischémiques transitoires (AIT)
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Une méthode simple pour quantifier objectivement la force musculaire des rats et souris et l'effet de drogues, toxines, maladies musculaires (ex: myopathie) et neurodégénératives. Cette mesure de force est souvent employée en association avec le test de coordination motrice ROTAROD: un sujet présentant une coordination normale montrera des résultats médiocres en cas de faible force musculaire. Un must pour vos recherches sur l'activité, la coordination et le contrôle musculaire: particulièrement utile pour vos études sur les maladies de Parkinson et Huntington.
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