Publications

Dernière publication 27/11/2012

Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via ex

Background In the orofacial region, limited information is available concerning pathological tongue pain, such as inflammatory pain or...

Array
(
    [id_prestablog_news] => 445
    [id_shop] => 1
    [date] => 2012-11-27 00:00:00
    [date_modification] => 2024-02-09 14:15:11
    [langues] => ["1","2"]
    [actif] => 1
    [slide] => 0
    [url_redirect] => 
    [average_rating] => 
    [number_rating] => 
    [author_id] => 1
    [featured] => 0
    [ishero] => 0
    [prim_key] => 876
    [id_lang] => 2
    [title] => Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via ex
    [paragraph] => Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via extracellular signalregulated kinase signaling in the trigeminal spinal subnucleus caudalis and upper cervical spinal cord
    [content] => 
Authors
M.-G. Liu, S. Matsuura, M. Shinoda, K. Honda, I. Suzuki et al.

 Lab
Nihon University School of Dentistry, Tokyo, Japan.
Journal
Journal of Neuroinflammation
Abstract
Background
 In the orofacial region, limited information is available concerning pathological tongue pain, such as inflammatory pain or neuropathic pain occurring in the tongue. Here, we tried for the first time to establish a novel animal model of inflammatory tongue pain in rats and to investigate the roles of metabotropic glutamate receptor 5 (mGluR5)-extracellular signalregulated kinase (ERK) signaling in this process.
 Methods
 Complete Freund’s adjuvant (CFA) was submucosally injected into the tongue to induce the inflammatory pain phenotype that was confirmed by behavioral testing. Expression of phosphorylated ERK (pERK) and mGluR5 in the trigeminal subnucleus caudalis (Vc) and upper cervical spinal cord (C1-C2) were detected with immunohistochemical staining and Western blotting. pERK inhibitor, a selective mGluR5 antagonist or agonist was continuously administered for 7 days via an intrathecal (i.t.) route. Local inflammatory responses were verified by tongue histology.
 Results
 Submucosal injection of CFA into the tongue produced a long-lasting mechanical allodynia and heat hyperalgesia at the inflamed site, concomitant with an increase in the pERK immunoreactivity in the Vc and C1-C2. The distribution of pERK-IR cells was laminar specific, ipsilaterally dominant, somatotopically relevant, and rostrocaudally restricted. Western blot analysis also showed an enhanced activation of ERK in the Vc and C1-C2 following CFA injection. Continuous i.t. administration of the pERK inhibitor and a selective mGluR5 antagonist significantly depressed the mechanical allodynia and heat hyperalgesia in the CFA-injected tongue. In addition, the number of pERK-IR cells in ipsilateral Vc and C1-C2 was also decreased by both drugs. Moreover, continuous i.t. administration of a selectivemGluR5 agonist induced mechanical allodynia in naive rats.
Conclusions
The present study constructed a new animal model of inflammatory tongue pain in rodents,and demonstrated pivotal roles of the mGluR5-pERK signaling in the development ofmechanical and heat hypersensitivity that evolved in the inflamed tongue. This tongueinflamedmodel might be useful for future studies to further elucidate molecular and cellular mechanisms of pathological tongue pain such as burning mouth syndrome.
BIOSEB Instruments Used
Electronic Von Frey 4 (BIO-EVF4),Electronic Von Frey 5 with embedded camera (BIO-EVF5)

Keywords/Topics
Douleurs inflammatoires; Douleur
    [meta_description] => 
    [meta_keywords] => http://www.jneuroinflammation.com/content/pdf/1742-2094-9-258.pdf
    [meta_title] => 
    [link_rewrite] => metabotropic-glutamate-receptor-5-contributes-to-inflammatory-tongue-pain-via-extracellular-signalregulated-kinase-signaling-in-the-trigeminal-spinal-subnucleus-caudalis-and-upper-cervical-spinal-cord
    [actif_langue] => 1
    [read] => 959
    [count_comments] => 0
    [id] => 445
    [categories] => Array
        (
            [10] => Array
                (
                    [id_prestablog_categorie] => 10
                    [title] => Douleur
                    [link_rewrite] => Douleur
                )

            [28] => Array
                (
                    [id_prestablog_categorie] => 28
                    [title] => Douleurs inflammatoires
                    [link_rewrite] => Douleurs-inflammatoires
                )

            [2] => Array
                (
                    [id_prestablog_categorie] => 2
                    [title] => Publications
                    [link_rewrite] => publications
                )

        )

    [authors] => 
    [paragraph_crop] => Metabotropic glutamate receptor 5 contributes to inflammatory tongue pain via extracellular [...]
    [link_for_unique] => 1
    [products_liaison] => Array
        (
            [1859] => Array
                (
                    [name] => Von Frey Électronique - Sans Fil
                    [description_short] => 
Une solution rapide pour déterminer le seuil de sensibilité à la douleur mécanique chez les rongeurs (rats et souris). Maintenant en version sans-fil, pour être libéré des câbles!

Cet instrument électronique précis et simple d'utilisation est le test de référence pour vos recherches en analgésie, nociception, neuropathies et douleurs post-opératoires

                    [thumb] => 
                    [img_empty] => /var/www/vhosts/de3310.ispfr.net/bioseb2024/modules/prestablog/views/img/product_link_white.jpg
                    [image_presente] => 1
                    [link] => https://bioseb.com/fr/douleur-allodyniehyperalgesie-mecanique/1859-electronic-von-frey-4.html
                )

            [1860] => Array
                (
                    [name] => Von Frey Électronique 5 avec caméra intégrée
                    [description_short] => 

En tant que version électronique du classique esthésiomètre à filaments de Von Frey, la dernière évolution de l’instrument électronique Von Frey de Bioseb, conçu pour déterminer le seuil de sensibilité mécanique chez les rongeurs (rats et souris) par stimulation sous la patte, est un outil indispensable pour vos recherches sur l’hyperalgésie et l’allodynie. En mesurant et en enregistrant la force à laquelle l’animal présente un réflexe de retrait de la patte, il est possible d’étudier les pathologies liées à la réponse sensorielle ainsi qu’à l’hyper- ou l’hypoesthésie.
L'EVF5 intègre une caméra embarquée au niveau de la zone de stimulation et fonctionne avec un nouveau logiciel dédié révolutionnaire

                    [thumb] => 
                    [img_empty] => /var/www/vhosts/de3310.ispfr.net/bioseb2024/modules/prestablog/views/img/product_link_white.jpg
                    [image_presente] => 1
                    [link] => https://bioseb.com/fr/douleur-allodyniehyperalgesie-mecanique/1860-electronic-von-frey-5-with-embedded-camera.html
                )

        )

)
1