Regulation of osteoarthritis and associated anxiety-related behaviours by retinoic acid binding protein 2 and its interaction with the insular cortex

Authors
Yixuan Zhao, Qian Zhang, Fantao Meng, Qianqian Fan, Hongkai Qi, Hongzhen Du, Lihong Xu, Yifan Cao, Dan Wang, Gaocai Man, Chen Li, Wei Li


Lab

Journal
British Journal of Pharmacology

Abstract
Figure S4Overexpression of Crabp2 exacerbates OA and anxious phenotypes in female mice. (a) Schematic Diagram of the experiments and representative immunofluorescence images and percentage analysis showing co-localization of Crabp2 -GFP (green) with DAPI-stained nuclei (blue) in articular cartilage. Scale bars = 100 μm. (b) X-ray test (red circle indicating the OA side and black circle indicating control). (c) The Kellgren–Lawrence rating. (d) Grip test. (e) Representative tracking maps in Elevated Plus Maze (EPM) Test. (f) Open arm time of EPM. (g) Open arm entries of EPM. (h) Total arm entries of EPM. (i) Latency in LDB test. (j) Time in light side in LDB. (k) Transition count in LDB. (l) The heatmap in Open Field Test. (OF) (m) Time in centre in OF. Latency in LDB test. (n) Total distance in centre in OF. (o) Total distance in OF. (p) Locomotor activity with total distance. (q) Locomotor activity with total distance analysis. (r)SO&FG staining shows articular cartilage wear, bone redundancy reduction, and osteophytes in knee joints. (s) Quantification of OARSI score. (t) cartilage area. (u) osteophyte score. (v) The mRNA levels of TNFα. (w) The mRNA levels of IL-1β. (x) The mRNA levels of IL-4. (y)The mRNA levels of Crabp2. (z) Immunoblot of articular cartilage protein levels: MMP3, MMP13, and ADAMTS5. (aa) Analysis of articular cartilage protein levels: MMP3. (ab) Analysis of articular cartilage protein levels: MMP13. (ac) Analysis of articular cartilage protein levels: ADAMTS5. (a) n = 5 animals per group, (c-q, v-y) n = 8 animals per group, (r-u, z-ac) n = 6 animals per group. *P< 0.05, *P< 0.01.

Keywords/Topics
osteoarthritis; anxiety; crabp2; insular cortex; knee-brain axis

BIOSEB Instruments Used:
Grip strength test (BIO-GS4)

Source :

https://bpspubs.onlinelibrary.wiley.com/doi/abs/10.1111/bph.70224

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