Automated 3D Open Field for Rats and Mice

Presentation

Bioseb developed a very innovative technology for automation of the OPEN FIELD test. The Open Field is one of the most widely used test to evaluate the anxiolytic effects of pharmacological substances or for phenotyping.

Although the Open field test is the most widely used test to evaluate basal exploratory behavior, none of the currently available systems can record horizontal and vertical activity without adding a third component such as beam break frames. BIOSEB OF3C utilizes 3D video technology to achieve 100% rearing detection by direct height measurements.

The 3D Open Field OF-3C is a dedicated software solution including the hardware infrastructure necessary for quickly analysing spontaneous locomation of a rodent in a new environment.

Operating principle

OF3C Screenshot

The OF-3C tracks a single subject (rat or mouse) with aerial video within the arena and records each rearing event based on the predefined height with an accuracy of 1 mm over 1 mm2. Our rearing detection algorithm perfectly matches manual scoring. Zone transitions remain realistic thanks to surface thresholds and specific morphologic detection. Drawing a zone is extremely easy using our predefined zone tools: name, color, form, layer priority for complex zone shapes, etc.

The straightforward software computes and stores data in real time. While it can be used with any type of arena for 2D detection, all components needed for 3D detection are provided with your purchase.