The joint protective function of live- and dead-Lactobacillus plantarum GKD7 on anterior cruciate ligament transection induces osteoarthritis

Authors
Yen-You Lin, Chih-Ying, Wu, You-Shan Tsai, Chin-Chu Chen, Tzu-Ching Chang, Li-Chai Chen, Hsien-Te Chen, Chin-Jung Hsu, Chih-Hsin Tang


Lab

Journal
Aging (Albany NY)

Abstract
Chondroprotective effects are the main issue of the OA management. The matrix degradation of the cartilage is the major characteristic of OA, and MMP3 play the initiates which will be secreted by synovial cell and chondrocytes to promote MMPs degrading matrix of cartilage, such as collage II and aggrecan [47-49], synovial membrane hyperplasia, and promote osteoclast mature. Moreover, MMP3 also facilitate inflammatory cell accumulation, vascular invasion on bone and synovium and inhibit mesenchymal stem cell differentiation of OA [47]. Prior research has demonstrated that MMP3 is a member of the MMPs family, which is able to break down different cartilage matrix constituents. Furthermore, MMP3 has been identified as an important OA marker. According to mechanistic studies, MMP3 starts MMP-mediated degradation events in OA and acts as an activator for several other pro-MMPs. As a result, our main objective was to evaluate MMP3 expression in joint tissue [47]. The previous study also demonstrated that MMP3 inhibitor improves the progression of OA with reducing collage II degradation [50]. Moreover, the previous study also reported that MMP have been considered as important mark and function in synovial inflammation, which can degrade extracellular matrix with remodeling processes of arthritic disease [50]. Thus, MMP3 also have been considered as the important target in discovery for OA therapeutic strategy. In the present study, we also observe the high expression of MMP3 on ACLT-induced rat, and oral administration of GKD7-L and GKD7-D both significantly decrease the MMP3 expression with reversing the collage II and aggrecan degradation. Thus, GKD7 show the chondroprotective effects through inhibiting MMP3 activity with preventing collage II and aggrecan degradation in OA.

Keywords/Topics
osteoarthritis ( OA ) ; Lactobacillus plantarum GKD7 ; metalloproteinase ( MMP ) 3 ;collage II ;aggrecan

BIOSEB Instruments Used:
Static Weight Bearing Touch: Incapacitance Test (BIO-SWB-TOUCH-M)

Source :

https://pmc.ncbi.nlm.nih.gov/articles/PMC11466490/

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