Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice- Multifaceted intervention against hyperglycemia- oxidative stress and depression

Authors
Wen Song, Ronge Xing, Haoyue Yang, Song Liu, Huahua Yu, Pengcheng Li


Lab

Journal
Journal of the Science of Food and Agriculture

Abstract
Our study employed an enzymatic approach to extract eumelanin from squid ink and characterized it using spectroscopic techniques. Remarkably, eumelanin extracted with alkaline-neutral-flavor protease (ANF) displayed superior inhibitory activity againstAlpha-glucosidase andAlpha-amylase, while enhancing glucose utilization and hepatic glycogen synthesis in human hepatocellular carcinoma cell line (HepG2) insulin resistance model. Further evaluation of ANF in a T2DM ICR mouse model demonstrated its significant potential in alleviating hyperglycemia, reducing glycosylated serum protein levels, improving glucose tolerance and modulating total cholesterol and low-density lipoprotein levels, as well as antioxidant indices at a dosage of 0.04gkgto1. Additionally, ANF exhibited positive effects on energy levels and reduced immobility time in antidepressant behavioral experiments. Moreover, ANF positively influenced the density and infiltration state of renal cells, while mitigating inflammatory enlargement and deformation of liver cells, without inducing any adverse effects in mice.

Keywords/Topics
therapeutic;potential;enzymatically;extracted;eumelanin;squid;diabetes;mellitus;multifaceted;intervention

BIOSEB Instruments Used:
Tail Suspension Test - Wireless (BIO-TST5)

Source :

https://scijournals.onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.12986

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