Self-Assembling Hydrogel for Controlled Release of bFGF and Ang-1 Mimetic Peptide Promotes Neurovascular Repair in Ischemic Stroke Rats

Authors
Ange Nie, Weihong Nie, Na Zhou, Ran Li, Rui Zhang, Chunying Shi, Haicheng Yuan


Lab

Journal
Journal of Biomedical Materials Research Part A

Abstract
Self-assembling peptide hydrogels represent a promising strategy for growth factor delivery and tissue repair, with the advantage of sustained release and micro-environment response characteristics. In the present study, a multiple biofunctional self-assembling hydrogels-FGFP/TIMP-AMP was constructed, which comprised three key components: (1) a basic fibroblast growth factor-mimetic peptide (FGFP), (2) an angiopoietin-1-mimetic peptide (AMP), and (3) a microenvironment-responsive TIMP sequence designed to target MMP-2. The FGFP/TIMP-AMP could assemble into a hydrogel under physiological conditions with a suitable pore size for cell growth. In vitro, it significantly promoted HUVEC migration and tube formation. Using a PC12 deprivation of oxygen and glucose (OGD) model, it effectively reduced apoptosis and protected against hypoxia. Furthermore, the bFGF/TIMP-AMP assembling hydrogels enhanced neuronal survival, vascular regeneration, and blood–brain barrier (BBB) repair in a middle cerebral artery occlusion (MCAO) rat model. These morphological improvements further promoted the recovery of motor function in behavioral tests. Therefore, these results highlighted the therapeutic potential of FGFP/TIMP-AMP assembling hydrogel for the repair of ischemic stroke.

Keywords/Topics
blood–brain barrier; cerebral ischemia/reperfusion injury; growth factor delivery; self-assembling nanofibrous hydrogel; synergistic effect

Source :

https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.70063

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