Phenotyping - page 3 Scientific Publications

Latest publication 07/30/2007

Analysis of dopamine transporter gene expression pattern _ generation of DAT-iCr

The dopamine transporter is an essential component of the dopaminergic synapse. It is located in the presynaptic neurons and regulates...

Array
(
    [id_prestablog_news] => 118
    [id_shop] => 1
    [date] => 2007-07-30 00:00:00
    [date_modification] => 2024-02-09 14:15:11
    [langues] => ["1","2"]
    [actif] => 1
    [slide] => 0
    [url_redirect] => 
    [average_rating] => 
    [number_rating] => 
    [author_id] => 1
    [featured] => 0
    [prim_key] => 233
    [id_lang] => 1
    [title] => Analysis of dopamine transporter gene expression pattern _ generation of DAT-iCr
    [paragraph] => Analysis of dopamine transporter gene expression pattern _ generation of DAT-iCre transgenic mice.
    [content] => 
Authors
M. Turiault, S. Parnaudeau, A. Milet, R. Parlato, J.-D. Rouzeau et al.

 Lab
Collège de France, Institut de Biologie, Neurophysiology and Behavior, CNRS UMR7148, Molecular Genetics, Paris, France.
Journal
FEBS Journal
Abstract
The dopamine transporter is an essential component of the dopaminergic synapse. It is located in the presynaptic neurons and regulates extracellular dopamine levels. We generated a transgenic mouse line expressing the Cre recombinase under the control of the regulatory elements of the dopamine transporter gene, for investigations of gene function in dopaminergic neurons. The codon-improved Cre recombinase (iCre) gene was inserted into the dopamine transporter gene on a bacterial artificial chromosome. The pattern of expression of the bacterial artificial chromosome–dopamine transporter–iCre transgene was similar to that of the endogenous dopamine transporter gene, as shown by immunohistochemistry. Recombinase activity was further studied in mice carrying both the bacterial artificial chromosome–dopamine transporter–iCre transgene and a construct expressing the _-galactosidase gene after Cre-mediated recombination. In situ studies showed that _-galactosidase (5-bromo-4-chloroindol-3-yl _-d-galactoside staining) and the dopamine transporter (immunofluorescence) had identical distributions in the ventral midbrain. We used this animal model to study the distribution of dopamine transporter gene expression in hypothalamic nuclei in detail. The expression profile of tyrosine hydroxylase (an enzyme required for dopamine synthesis) was broader than that of _-galactosidase in A12 to A15. Thus, only a fraction of neurons synthesizing dopamine expressed the dopamine transporter gene. The bacterial artificial chromosome–dopamine transporter–iCre transgenic line is a unique tool for targeting Cre/loxP-mediated DNA recombination to dopamine neurons for studies of gene function or for labeling living cells, following the crossing of these mice with transgenic Cre reporter lines producing fluorescent proteins.
BIOSEB Instruments Used
Grip strength test (BIO-GS3)
    [meta_description] => 
    [meta_keywords] => http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2007.05886.x/full
    [meta_title] => 
    [link_rewrite] => analysis-of-dopamine-transporter-gene-expression-pattern--generation-of-dat-icre-transgenic-mice-
    [actif_langue] => 1
    [read] => 161
    [count_comments] => 0
    [id] => 118
    [categories] => Array
        (
            [24] => Array
                (
                    [id_prestablog_categorie] => 24
                    [title] => Cross-disciplinary subjects 
                    [link_rewrite] => Cross-disciplinary-subjects-
                )

            [87] => Array
                (
                    [id_prestablog_categorie] => 87
                    [title] => Phenotyping
                    [link_rewrite] => Phenotyping
                )

            [2] => Array
                (
                    [id_prestablog_categorie] => 2
                    [title] => Publications
                    [link_rewrite] => publications
                )

        )

    [authors] => 
    [paragraph_crop] => Analysis of dopamine transporter gene expression pattern _ generation of DAT-iCre transgenic mice.
    [link_for_unique] => 1
    [products_liaison] => Array
        (
            [48] => Array
                (
                    [name] => Grip strength test
                    [description_short] => 
An easy way to objectively quantify the muscular strength of mice and rats, and to assess the effect of drugs, toxins, muscular (i.e. myopathy) and neurodegenerative diseases on muscular degeneration. It is widely used in conjunction with the ROTAROD motor coordination test: a normally coordinated rodent will show a decreased latency to fall off the rotating rod if its muscular strength is low. The Grip Strength Test is a must for your research on activity, motor control & coordination, and is particularly well suited for studies on Parkinson's & Huntington's disease.
New features GS4 - 2023: Color display with permanent backlight screen for easier reading, reset by footswitch, Improved battery time, Larger data memory of 500 values, Animal counter, USB port (charging/data transfer)

                    [thumb] => 
                    [img_empty] => /var/www/vhosts/de3310.ispfr.net/bioseb2024/modules/prestablog/views/img/product_link_white.jpg
                    [image_presente] => 1
                    [link] => https://bioseb.com/en/activity-motor-control-coordination/48-grip-strength-test.html
                )

        )

)
1