Pain - page 14 Scientific Publications

Latest publication 05/29/2013

Mature Purkinje Cells Require the Retinoic Acid-Related Orphan Receptor-α -

Neuronal maturation during development is a multistep process regulated by transcription factors. The transcription factor RORα (retinoic...

Array
(
    [id_prestablog_news] => 490
    [id_shop] => 1
    [date] => 2013-05-29 00:00:00
    [date_modification] => 2024-02-09 14:15:11
    [langues] => ["1","2"]
    [actif] => 1
    [slide] => 0
    [url_redirect] => 
    [average_rating] => 
    [number_rating] => 
    [author_id] => 1
    [featured] => 0
    [prim_key] => 961
    [id_lang] => 1
    [title] => Mature Purkinje Cells Require the Retinoic Acid-Related Orphan Receptor-α -
    [paragraph] => Mature Purkinje Cells Require the Retinoic Acid-Related Orphan Receptor-α (RORα) to Maintain Climbing Fiber Mono-Innervation and Other Adult Characteristics 
    [content] => 
Authors
XR.Chen , N. Heck, AM. Lohof, C. Rochefort, MP. More et al

 Lab
UPMC Université Paris 06, CNRS, UMR 7102, Paris, France,
Journal
The Journal of Neuroscience
Abstract
Neuronal maturation during development is a multistep process regulated by transcription factors. The transcription factor RORα (retinoic acid-related orphan receptor α) is necessary for early Purkinje cell (PC) maturation but is also expressed throughout adulthood. To identify the role of RORα in mature PCs, we used Cre-lox mouse genetic tools in vivo that delete it specifically from PCs between postnatal days 10–21. Up to 14 d of age, differences between mutant and control PCs were not detectable: both were mono-innervated by climbing fibers (CFs) extending along their well-developed dendrites with spiny branchlets. By week 4, mutant mice were ataxic, some PCs had died, and remaining PC soma and dendrites were atrophic, with almost complete disappearance of spiny branchlets. The innervation pattern of surviving RORα-deleted PCs was abnormal with several immature characteristics. Notably, multiple functional CF innervation was reestablished on these mature PCs, simultaneously with the relocation of CF contacts to the PC soma and their stem dendrite. This morphological modification of CF contacts could be induced even later, using lentivirus-mediated depletion of rora from adult PCs. These data show that the late postnatal expression of RORα cell-autonomously regulates the maintenance of PC dendritic complexity, and the CF innervation status of the PC (dendritic vs somatic contacts, and mono-innervation vs multi-innervation). Thus, the differentiation state of adult neurons is under the control of transcription factors; and in their absence, adult neurons lose their mature characteristics and acquire some characteristics of an earlier developmental stage. 
BIOSEB Instruments Used
Aron Test or Four Plates Test (LE830),Rotarod (BX-ROD)
    [meta_description] => 
    [meta_keywords] => http://www.jneurosci.org/content/33/22/9546.short
    [meta_title] => 
    [link_rewrite] => mature-purkinje-cells-require-the-retinoic-acid-related-orphan-receptor-945--ror945--to-maintain-climbing-fiber-mono-innervation-and-other-adult-characteristics-
    [actif_langue] => 1
    [read] => 166
    [count_comments] => 0
    [id] => 490
    [categories] => Array
        (
            [29] => Array
                (
                    [id_prestablog_categorie] => 29
                    [title] => Neuropathic pain
                    [link_rewrite] => Neuropathic-pain
                )

            [10] => Array
                (
                    [id_prestablog_categorie] => 10
                    [title] => Pain
                    [link_rewrite] => Pain
                )

            [2] => Array
                (
                    [id_prestablog_categorie] => 2
                    [title] => Publications
                    [link_rewrite] => publications
                )

        )

    [authors] => 
    [paragraph_crop] => Mature Purkinje Cells Require the Retinoic Acid-Related Orphan Receptor-α (RORα) to [...]
    [link_for_unique] => 1
    [products_liaison] => Array
        (
            [588] => Array
                (
                    [name] => Aron Test or Four Plates Test
                    [description_short] => 
The Four Plates Test is an exclusivity from BIOSEB!
The Aron test allows a quick characterization of putative anxiolytics compounds in naïve animals. A must for studying anxiety, drug screening and phenotyping.
                    [thumb] => 
                    [img_empty] => /var/www/vhosts/de3310.ispfr.net/bioseb2024/modules/prestablog/views/img/product_link_white.jpg
                    [image_presente] => 1
                    [link] => https://bioseb.com/en/anxiety-depression-disorder/588-aron-test-or-four-plates-test.html
                )

            [1326] => Array
                (
                    [name] => Rotarod for rats and mice
                    [description_short] => 
Rotarod provides an easy way to test the motor activity in rodents (mouse or rat) - an ideal solution for studying central nervous system damage, disease effects on motor activity, drugs administration, fatigue resistance, etc. Now even easier to use! The new touchscreen graphic user interface allows clear visualization of timing and speed for each lane. Change modes, adjust speed, and create protocols right from the main screen for greater flexibility with maximum functionality and usability

                    [thumb] => 
                    [img_empty] => /var/www/vhosts/de3310.ispfr.net/bioseb2024/modules/prestablog/views/img/product_link_white.jpg
                    [image_presente] => 1
                    [link] => https://bioseb.com/en/activity-motor-control-coordination/1326-rotarod.html
                )

        )

)
1